OBJECTIVE: Fetal cells from the maternal circulation (FCMBs) have the potential to replace cells from amniotic fluid or chorionic villi in a diagnosis of common chromosomal aneuploidies. Good markers for enrichment and identification are lacking. METHOD: Blood samples from 78 normal pregnancies were used for testing the marker-set CD105 and CD141 for fetal cell enrichment. Fetal cell candidates were subsequently stained by a cocktail of cytokeratin antibodies, and the gender of the fetal cells was explored by fluorescence in situ hybridization (FISH) of the X and Y chromosomes. RESULTS: Fetal cell candidates could be detected in 91% of the samples, and in 85% of the samples, it was possible to obtain X and Y chromosomal FISH results for gender determination. The concordance between gender determined by FISH on fetal cells in maternal blood and gender found at birth reached 100% if three or more fetal cells with FISH signals could be found in a sample. CONCLUSION: The marker set identifies fetal cells with specificity high enough to make cell-based noninvasive prenatal diagnosis realistic.
OBJECTIVE: Fetal cells from the maternal circulation (FCMBs) have the potential to replace cells from amniotic fluid or chorionic villi in a diagnosis of common chromosomal aneuploidies. Good markers for enrichment and identification are lacking. METHOD: Blood samples from 78 normal pregnancies were used for testing the marker-set CD105 and CD141 for fetal cell enrichment. Fetal cell candidates were subsequently stained by a cocktail of cytokeratin antibodies, and the gender of the fetal cells was explored by fluorescence in situ hybridization (FISH) of the X and Y chromosomes. RESULTS: Fetal cell candidates could be detected in 91% of the samples, and in 85% of the samples, it was possible to obtain X and Y chromosomal FISH results for gender determination. The concordance between gender determined by FISH on fetal cells in maternal blood and gender found at birth reached 100% if three or more fetal cells with FISH signals could be found in a sample. CONCLUSION: The marker set identifies fetal cells with specificity high enough to make cell-based noninvasive prenatal diagnosis realistic.
Authors: Jacob Mørup Schlütter; Ida Kirkegaard; Olav Bjørn Petersen; Nanna Larsen; Britta Christensen; David M Hougaard; Steen Kølvraa; Niels Uldbjerg Journal: PLoS One Date: 2014-09-04 Impact factor: 3.240
Authors: Anne van de Looij; Ripudaman Singh; Lotte Hatt; Katarina Ravn; Line D Jeppesen; Bolette H Nicolaisen; Mathias Kølvraa; Ida Vogel; Palle Schelde; Niels Uldbjerg Journal: Acta Obstet Gynecol Scand Date: 2020-05-17 Impact factor: 3.636
Authors: Amy M Breman; Jennifer C Chow; Lance U'Ren; Elizabeth A Normand; Sadeem Qdaisat; Li Zhao; David M Henke; Rui Chen; Chad A Shaw; Laird Jackson; Yaping Yang; Liesbeth Vossaert; Rachel H V Needham; Elizabeth J Chang; Daniel Campton; Jeffrey L Werbin; Ron C Seubert; Ignatia B Van den Veyver; Jackie L Stilwell; Eric P Kaldjian; Arthur L Beaudet Journal: Prenat Diagn Date: 2016-10-02 Impact factor: 3.050