Manar Samman1, Henry Wood1, Caroline Conway1, Stefano Berri1, Monica Pentenero2, Sergio Gandolfo2, Adele Cassenti3, Paola Cassoni3, Abdulaziz Al Ajlan4, A William Barrett5, Preetha Chengot6, Kenneth MacLennan1, Alec S High7, Pamela Rabbitts1. 1. Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, United Kingdom. 2. Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Turin, Italy. 3. Department of Medical Sciences, Pathology Unit, University of Turin, Turin, Italy. 4. Department of Pathology and Laboratory Medicine, National Guard Hospitals, Riyadh, Saudi Arabia. 5. Department of Histopathology, Queen Victoria Hospital, East Grinstead, United Kingdom. 6. Department of Histopathology, St James's University Hospital and Leeds Dental Institute, University of Leeds, Leeds, United Kingdom. 7. Department of Histopathology, St James's University Hospital and Leeds Dental Institute, University of Leeds, Leeds, United Kingdom. Electronic address: a.s.high@leeds.ac.uk.
Abstract
OBJECTIVE: The etiology of oral verrucous carcinoma is unknown, and human papillomavirus 'involvement' remains contentious. The uncertainty can be attributed to varied detection procedures and difficulties in defining 'gold-standard' histologic criteria for diagnosing 'verrucous' lesions. Their paucity also hampers investigation. We aimed to analyze oral verrucous lesions for human papillomavirus (HPV) subtype genomes. STUDY DESIGN: We used next-generation sequencing for the detection of papillomavirus sequences, identifying subtypes and computing viral loads. We identified a total of 78 oral verrucous cases (62 carcinomas and 16 hyperplasias). DNA was extracted from all and sequenced at a coverage between 2.5% and 13%. RESULTS: An HPV-16 sequence was detected in 1 carcinoma and 1 hyperplasia, and an HPV-2 sequence was detected in 1 carcinoma out of the 78 cases, with viral loads of 2.24, 8.16, and 0.33 viral genomes per cell, respectively. CONCLUSIONS: Our results indicate no conclusive human papillomavirus involvement in oral verrucous carcinoma or hyperplasia.
OBJECTIVE: The etiology of oral verrucous carcinoma is unknown, and human papillomavirus 'involvement' remains contentious. The uncertainty can be attributed to varied detection procedures and difficulties in defining 'gold-standard' histologic criteria for diagnosing 'verrucous' lesions. Their paucity also hampers investigation. We aimed to analyze oral verrucous lesions for human papillomavirus (HPV) subtype genomes. STUDY DESIGN: We used next-generation sequencing for the detection of papillomavirus sequences, identifying subtypes and computing viral loads. We identified a total of 78 oral verrucous cases (62 carcinomas and 16 hyperplasias). DNA was extracted from all and sequenced at a coverage between 2.5% and 13%. RESULTS: An HPV-16 sequence was detected in 1 carcinoma and 1 hyperplasia, and an HPV-2 sequence was detected in 1 carcinoma out of the 78 cases, with viral loads of 2.24, 8.16, and 0.33 viral genomes per cell, respectively. CONCLUSIONS: Our results indicate no conclusive human papillomavirus involvement in oral verrucous carcinoma or hyperplasia.
Authors: Beatriz Vera-Sirera; Luis Rubio-Martínez; Leopoldo Forner-Navarro; Francisco Vera-Sempere Journal: Head Face Med Date: 2018-07-13 Impact factor: 2.151