Zhifeng He1, Xiaofeng Huang1, Yanhong Ni1, Peihua Shi2, Zhiyong Wang2, Wei Han2, Qingang Hu3. 1. Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China. 2. Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China. 3. Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing, China. Electronic address: Qghu@nju.edu.cn.
Abstract
OBJECTIVE: The aim of this study was to investigate the expression and function of toll-like receptor 3 (TLR3) in oral squamous cell carcinoma (OSCC). STUDY DESIGN: We first assessed TLR3 expression in 20 cases of primary OSCC tissue. Two OSCC cell lines, SCC4 and CAL27, were used for further study. Lyophilized polyinosinic-polycytidylic acid [Poly(I:C)] was used to activate TLR3 expressed by OSCC. Changes in cytokines expression, cell viability, apoptosis, and migration in OSCC were investigated. RESULTS: TLR3 was present in both OSCC tissue and the 2 OSCC cell lines examined. Poly(I:C) stimulated robust responses in OSCC: it upregulated cytokine expression; decreased cell viability by suppressing cell proliferation and inducing apoptosis; and decreased cell migration. Poly(I:C)-TLR3-induced OSCC cell apoptosis was caspase-3-dependent. CONCLUSIONS: The present study indicated that TLR3 might affect OSCC development and should be considered as a potential target for future OSCC immunotherapy.
OBJECTIVE: The aim of this study was to investigate the expression and function of toll-like receptor 3 (TLR3) in oral squamous cell carcinoma (OSCC). STUDY DESIGN: We first assessed TLR3 expression in 20 cases of primary OSCC tissue. Two OSCC cell lines, SCC4 and CAL27, were used for further study. Lyophilized polyinosinic-polycytidylic acid [Poly(I:C)] was used to activate TLR3 expressed by OSCC. Changes in cytokines expression, cell viability, apoptosis, and migration in OSCC were investigated. RESULTS:TLR3 was present in both OSCC tissue and the 2 OSCC cell lines examined. Poly(I:C) stimulated robust responses in OSCC: it upregulated cytokine expression; decreased cell viability by suppressing cell proliferation and inducing apoptosis; and decreased cell migration. Poly(I:C)-TLR3-induced OSCC cell apoptosis was caspase-3-dependent. CONCLUSIONS: The present study indicated that TLR3 might affect OSCC development and should be considered as a potential target for future OSCC immunotherapy.
Authors: Angela A Alexander-Bryant; Anca Dumitriu; Christopher C Attaway; Hong Yu; Andrew Jakymiw Journal: J Control Release Date: 2015-09-18 Impact factor: 9.776
Authors: Hector F Pelaez-Prestel; Jose L Sanchez-Trincado; Esther M Lafuente; Pedro A Reche Journal: Int J Mol Sci Date: 2021-11-10 Impact factor: 5.923