| Literature DB >> 24908186 |
Shamlan M S Reshamwala1, Sandip K Pagar2, Vishal S Velhal2, Vijay M Maranholakar2, Vishal G Talangkar2, Arvind M Lali3.
Abstract
Mannitol is a six carbon sugar alcohol that finds applications in the pharmaceutical and food industries. A novel Escherichia coli strain capable of converting D-glucose to D-mannitol has been constructed, wherein native mannitol-1-phosphate dehydrogenase (MtlD) and codon-optimized Eimeria tenella mannitol-1-phosphatase (M1Pase) have been overexpressed. Codon-optimized Pseudomonas stutzeri phosphite dehydrogenase (PtxD) was overexpressed for cofactor (NADH) regeneration with the concomitant oxidation of phosphite to phosphate. Whole-cell biotransformation using resting cells in a medium containing D-glucose and equimolar sodium phosphite resulted in d-mannitol yield of 87 mol%. Thus, production of an industrially relevant biochemical without using complex media components and elaborate process control mechanisms has been demonstrated.Entities:
Keywords: Biotransformation; Cofactor regeneration; Resting cells; Whole-cell biocatalyst; d-Mannitol production
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Year: 2014 PMID： 24908186 DOI： 10.1016/j.jbiosc.2014.05.004
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894