Mahmoud Aarabi1, Hanna Balakier2, Siamak Bashar2, Sergey I Moskovtsev3, Peter Sutovsky4, Clifford L Librach3, Richard Oko5. 1. Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, Ontario, Canada. 2. CReATe Fertility Centre, Toronto, Ontario, Canada. 3. CReATe Fertility Centre, Toronto, Ontario, Canada; Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada. 4. Division of Animal Sciences, University of Missouri, Columbia, Missouri; Departments of Obstetrics, Gynecology, and Women's Health, School of Medicine, University of Missouri, Columbia, Missouri. 5. Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, Ontario, Canada. Electronic address: ro3@queensu.ca.
Abstract
OBJECTIVE: To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. DESIGN: Prospective clinical and laboratory study. SETTING: University-based laboratory and infertility clinic. PATIENT(S): Men undergoing ICSI for the treatment of couples' infertility (n=110). INTERVENTION(S): Quantitative analysis of sperm PAWP levels by flow cytometry and developmental analysis of PAWP expression by immunoblotting, immunofluorescence, and immunohistochemistry. MAIN OUTCOME MEASURE(S): PAWP flow-cytometric levels and immunolocalization in spermatozoa. RESULT(S): A strong positive correlation was found between PAWP expression levels and fertilization rates after ICSI, with high levels of PAWP being associated with higher fertilization rates; the positive correlation was independent of age, DNA fragmentation index, and other sperm parameters. PAWP expression levels were correlated with embryonic development, with high levels of PAWP being associated with a lower number of arrested embryos within 3-5 days post-ICSI. PAWP expression was detected during the late stages of human spermiogenesis in elongating spermatids, confirming previous findings in various animal models. CONCLUSION(S): Our clinical data from infertile couples demonstrate significant correlations between sperm PAWP levels and both fertilization rates and normal embryonic development after ICSI. Considering its proposed role in the initiation of oocyte activation, we suggest that PAWP could have potential applications in the diagnosis and treatment of infertility.
OBJECTIVE: To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. DESIGN: Prospective clinical and laboratory study. SETTING: University-based laboratory and infertility clinic. PATIENT(S): Men undergoing ICSI for the treatment of couples' infertility (n=110). INTERVENTION(S): Quantitative analysis of sperm PAWP levels by flow cytometry and developmental analysis of PAWP expression by immunoblotting, immunofluorescence, and immunohistochemistry. MAIN OUTCOME MEASURE(S): PAWP flow-cytometric levels and immunolocalization in spermatozoa. RESULT(S): A strong positive correlation was found between PAWP expression levels and fertilization rates after ICSI, with high levels of PAWP being associated with higher fertilization rates; the positive correlation was independent of age, DNA fragmentation index, and other sperm parameters. PAWP expression levels were correlated with embryonic development, with high levels of PAWP being associated with a lower number of arrested embryos within 3-5 days post-ICSI. PAWP expression was detected during the late stages of human spermiogenesis in elongating spermatids, confirming previous findings in various animal models. CONCLUSION(S): Our clinical data from infertile couples demonstrate significant correlations between sperm PAWP levels and both fertilization rates and normal embryonic development after ICSI. Considering its proposed role in the initiation of oocyte activation, we suggest that PAWP could have potential applications in the diagnosis and treatment of infertility.
Authors: George Anifandis; Christina I Messini; Konstantinos Dafopoulos; Alexandros Daponte; Ioannis E Messinis Journal: J Assist Reprod Genet Date: 2016-01-16 Impact factor: 3.412
Authors: Abdullah Kaya; Sule Dogan; Peter Vargovic; Naseer Ahmad Kutchy; Pablo Ross; Einko Topper; Richard Oko; Frans van der Hoorn; Peter Sutovsky; Erdogan Memili Journal: Cell Tissue Res Date: 2021-11-11 Impact factor: 5.249