| Literature DB >> 24906740 |
Peipei Wang1, Wenfeng Liao2, Jianping Fang2, Qin Liu2, Jian Yao2, Minghua Hu3, Kan Ding4.
Abstract
Inhibition of Aβ aggregation and attenuation of its cytotoxicity are considered to valuable therapeutics for Alzheimer's disease (AD). Here, a glucan named as LJW0F2 was purified from flowers of Lonicera japonica Thunb. Using monosaccharides composition analysis, methylation analysis, IR and NMR spectroscopy, this polysaccharide was elucidated to be an α-D-(1→4)-glucan with an α-(1→4) linked branch attached to the C-6 position. Its inhibitory effect on Aβ42 aggregation was measured by fluorescence spectroscopic analysis with thioflavine T (ThT) and atomic force microscopy (AFM). We showed that polysaccharide LJW0F2 could inhibit Aβ42 aggregation in a dose-dependent-manner. Besides, LJW0F2 could attenuate the cytotoxicity induced by Aβ42 aggregation in SH-SY5Y neuroblastoma cells. To the best of our knowledge, this was the first report that the exogenous plant-derived polysaccharide might block Aβ42 aggregation directly and reduce its toxicity in SH-SY5Y cells.Entities:
Keywords: Aggregation and neurotoxicity; Alzheimer's disease; Aβ(42); Glucan; Lonicera japonica Thunb.
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Year: 2014 PMID： 24906740 DOI： 10.1016/j.carbpol.2014.03.060
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381