| Literature DB >> 24906318 |
Naoko Yoshizawa-Sugata1, Hisao Masai.
Abstract
Analysis of cellular DNA content and measurement of pulse-labeled newly replicated DNA by flow cytometry are useful techniques for cell cycle studies. In this chapter, we describe the protocols for cell cycle synchronization of mammalian cells, including time course designs and consideration of cell types to achieve successful experiments, along with the methods for detection of DNA. Some selected applications dealing with siRNA-mediated knockdown are also presented.Entities:
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Year: 2014 PMID： 24906318 DOI： 10.1007/978-1-4939-0888-2_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745