Yi Lu1, Lin Zheng2, Wen Zhang3, Tingting Feng3, Juan Liu3, Xiao Wang4, Yuan Yu5, Mei Qi3, Weiming Zhao3, Xiuping Yu6, Wei Tang7. 1. Department of Medical Microbiology, Shandong University School of Medicine, Jinan, China; Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, China. 2. Department of Medical Microbiology, Shandong University School of Medicine, Jinan, China; Microbiological Lab, The Affiliated Hospital of School of Medicine of Ningbo University, Ningbo, China. 3. Department of Medical Microbiology, Shandong University School of Medicine, Jinan, China. 4. Department of Pathology, Shandong University School of Medicine, Jinan, China. 5. Department of Hematology, Qilu Hospital, Shandong University, Jinan, China. 6. Department of Medical Microbiology, Shandong University School of Medicine, Jinan, China. Electronic address: yuxp@sdu.edu.cn. 7. Department of Medical Microbiology, Shandong University School of Medicine, Jinan, China. Electronic address: weitang@sdu.edu.cn.
Abstract
OBJECTIVE: The growth factor progranulin (PGRN) is overexpressed in a number of tumors. We aimed to investigate the expression and role of PGRN in cervical cancer tumorigenesis. METHODS: PGRN expression and secretion was assessed in cells and normal and cancerous cervical tissues by Western blot analysis, ELISA or immunohistochemistry. The role of PGRN in cervical carcinogenesis was explored by cell-proliferation, colony-formation and tumor-growth assays. We assessed the role of PGRN-mediated signaling in the cervical cell with specific inhibitors. RESULTS: PGRN expression was upregulated in cervical cancer cell lines and tissue. PGRN promoted the transformation of human cervical mucosa epithelial H8 cells in vitro and tumor formation in vivo. Knockdown of PGRN expression in cervical cancer cells in vivo decreased cell proliferation and slowed tumor growth. PGRN stimulated cervical cell proliferation, and transformation was mediated, at least in part, by Akt and Erk signaling. CONCLUSIONS: PGRN is overexpressed in cervical cancer and promotes the malignant growth and transformation of cervical cells. Therefore, PGRN plays a critical role in carcinogenesis of cervical cancer and shows promise for therapeutic strategies for cervical cancer.
OBJECTIVE: The growth factor progranulin (PGRN) is overexpressed in a number of tumors. We aimed to investigate the expression and role of PGRN in cervical cancer tumorigenesis. METHODS:PGRN expression and secretion was assessed in cells and normal and cancerous cervical tissues by Western blot analysis, ELISA or immunohistochemistry. The role of PGRN in cervical carcinogenesis was explored by cell-proliferation, colony-formation and tumor-growth assays. We assessed the role of PGRN-mediated signaling in the cervical cell with specific inhibitors. RESULTS:PGRN expression was upregulated in cervical cancer cell lines and tissue. PGRN promoted the transformation of human cervical mucosa epithelial H8 cells in vitro and tumor formation in vivo. Knockdown of PGRN expression in cervical cancer cells in vivo decreased cell proliferation and slowed tumor growth. PGRN stimulated cervical cell proliferation, and transformation was mediated, at least in part, by Akt and Erk signaling. CONCLUSIONS:PGRN is overexpressed in cervical cancer and promotes the malignant growth and transformation of cervical cells. Therefore, PGRN plays a critical role in carcinogenesis of cervical cancer and shows promise for therapeutic strategies for cervical cancer.
Authors: Fabian Arechavaleta-Velasco; Carlos Eduardo Perez-Juarez; George L Gerton; Laura Diaz-Cueto Journal: Med Oncol Date: 2017-11-07 Impact factor: 3.064
Authors: Yi Pan; Siu Tim Cheung; Joanna Hung Man Tong; Ka Yee Tin; Wei Kang; Raymond Wai Ming Lung; Feng Wu; Hui Li; Simon Siu Man Ng; Tony Wing Chung Mak; Ka Fai To; Anthony Wing Hung Chan Journal: J Transl Med Date: 2018-06-04 Impact factor: 5.531