Meng-yu Gao1, Ling Chen1, Lu Yang1, Xiu Yu1, Jun-ping Kou2, Bo-yang Yu3. 1. State Key Laboratory of Natural Medicines, Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China. 2. State Key Laboratory of Natural Medicines, Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China. Electronic address: junpingkou@163.com. 3. State Key Laboratory of Natural Medicines, Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China. Electronic address: boyangyu59@163.com.
Abstract
BACKGROUND: Considering the key role of TF in coagulation of sepsis or acute lung injury (ALI), we investigated whether berberine (BBR) could inhibit TF expression and procoagulant activity and explored its possible mechanism. METHODS: The effects of berberine on the expression, procoagulant activity of TF and related signal pathways induced by lipopolysaccharide (LPS) were observed in THP-1 cells. RESULTS: Our results showed that berberine could inhibit LPS-induced TF activity and expression, and down-regulate NF-κB, Akt and MAPK/JNK/p38/ERK pathways. CONCLUSION: Berberine inhibits TF expression and related pathway, which provides some new insights on its mechanism for sepsis treatment.
BACKGROUND: Considering the key role of TF in coagulation of sepsis or acute lung injury (ALI), we investigated whether berberine (BBR) could inhibit TF expression and procoagulant activity and explored its possible mechanism. METHODS: The effects of berberine on the expression, procoagulant activity of TF and related signal pathways induced by lipopolysaccharide (LPS) were observed in THP-1 cells. RESULTS: Our results showed that berberine could inhibit LPS-induced TF activity and expression, and down-regulate NF-κB, Akt and MAPK/JNK/p38/ERK pathways. CONCLUSION:Berberine inhibits TF expression and related pathway, which provides some new insights on its mechanism for sepsis treatment.