Pushkar Kulkarni1, Girish Hari Chaudhari2, Vijaykumar Sripuram3, Rakesh Kumar Banote2, Krishna Tulasi Kirla2, Razia Sultana2, Pallavi Rao3, Srinivas Oruganti4, Kiranam Chatti5. 1. Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: pushkark@drils.org. 2. Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. 3. Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. 4. Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: soruganti@drils.org. 5. Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: kiranamc@drils.org.
Abstract
BACKGROUND AND METHODS: We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated the pharmacological anxiolytic effect using novel tank test. RESULTS: The PK study confirmed the presence of CBZ in both blood and brain and the behavioural study showed dose dependent anxiolytic effect. The reproducibility of oral dosing was confirmed by the fact that the results obtained in both the experiments had negligible errors. CONCLUSIONS: This report enables a novel approach for optimizing the utility of zebrafish in drug discovery and drug delivery research.
BACKGROUND AND METHODS: We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated the pharmacological anxiolytic effect using novel tank test. RESULTS: The PK study confirmed the presence of CBZ in both blood and brain and the behavioural study showed dose dependent anxiolytic effect. The reproducibility of oral dosing was confirmed by the fact that the results obtained in both the experiments had negligible errors. CONCLUSIONS: This report enables a novel approach for optimizing the utility of zebrafish in drug discovery and drug delivery research.
Authors: Darya A Meshalkina; Elana V Kysil; Jason E Warnick; Konstantin A Demin; Allan V Kalueff Journal: Lab Anim (NY) Date: 2017-10-06 Impact factor: 12.625
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Authors: Ainhoa Alzualde; Oihane Jaka; Diogo A R S Latino; Omar Alijevic; Iñaki Iturria; Jorge Hurtado de Mendoza; Pavel Pospisil; Stefan Frentzel; Manuel C Peitsch; Julia Hoeng; Kyoko Koshibu Journal: J Nat Med Date: 2021-07-15 Impact factor: 2.343