Draft Genome Sequence of Defluviimonas indica Strain 20V17T, Isolated from a Deep-Sea Hydrothermal Vent Environment in the Southwest Indian Ocean.

Here, we present the draft genome sequence of Defluviimonas indica 20V17(T), which was isolated from a deep-sea hydrothermal vent chimney sample in the southwest Indian Ocean. The draft genome sequence contains 4,268,338 bp, with a G+C content of 66.33%.

GENOME ANNOUNCEMENT

Defluviimonas indica 20V17T (CGMCC 1.10859 =JCM 17871 =MCCC 1A01802) was isolated from a hydrothermal sulfide chimney sample in the Southwest Indian Ridge (1). The genus Defluviimonas was first proposed by Foesel et al. (2), which classified it within the family Rhodobacteraceae. Currently, the genus contains only three species, while strain 20V17T is the first isolate from the deep-sea hydrothermal environment in the genus Defluviimonas.

The draft genome of 20V17T was sequenced by Illumina paired-end sequencing technology at Beijing Genomics Institute (BGI) (Shenzhen, China). The reads were assembled using SOAPdenovo (version 1.05), and 236 contigs were generated, with a maximum contig size of 155,100 bp and an N50 contig length of 39,530 bp. The assembled genome comprises 4,268,338 nucleotides with a G+C content of 66.33%. Gene annotation was carried out by the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (PGAAP). A total of 4,190 genes were predicted, including 4,119 protein-coding genes, 42 tRNAs, and 4 rRNAs.

D. indica 20V17T was able to oxidize reduced sulfur compounds as energy sources. RAST annotation also revealed that the draft genome contains sulfur oxidation genes (soxRSVWXYZABCD). Moreover, 11 genes encoding an [NiFe] hydrogenase were found in the draft sequence, indicating that strain 20V17T might also be able to use H2 as an electron donor. The metabolic plasticity of D. indica 20V17T, which uses both molecular hydrogen and reduced sulfur compounds as energy sources, might represent a very useful strategy for living in deep-sea hydrothermal vent systems. The draft genome sequence of 20V17T will enable further studies on the physiology and metabolic potential of this species, especially its roles involved in sulfur and H2 oxidation, which are major energy metabolisms in deep-sea hydrothermal vent environments.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited in DDBJ/EMBL/GenBank under the accession no. AYXI00000000. The version described in this paper is the first version, AYXI01000000.