PURPOSE: This study focuses on nanoscale self-assembly peptides (SAP) modified demineralized bone matrix (DBM) which provided a more effective osteogenesis and regeneration for critically-sized femur defects in goats using the selective cell retention (SCR) strategy. METHODS: RADA16-I peptide was used to modify DBM and formed a composite scaffold (SAP/DBM). The morphological change and dynamic expression of osteogenic genes of mesenchymal stem cells (MSCs) derived from marrow in SAP/DBM was observed. The cells and factors in bone marrow were enriched into SAP/DBM by technology of selective cells retension (SCR). The construct was transplanted into 20-mm femur defects in goats and their osteogenesis was evaluated. RESULTS: The SAP/DBM scaffold formed a three-dimensional interweaving nanofiber in pores of DBM. MSCs exhibited better morphology in SAP/DBM than that in only DBM, and the levels of expression of ALP ,OCN and Runx2 gene in SAP/DBM samples was significantly higher than that of DBM at 14 days in vitro (P < 0.05). Compared with marrow-enriched DBM, the volume of newly formed bone from marrow-enriched SAP/DBM is higher in goats (P < 0.05). CONCLUSION: Our study may not only have a significant impact on the construction method of tissue engineering but also provide a viable, simple and effective method for clinical bone construction.
PURPOSE: This study focuses on nanoscale self-assembly peptides (SAP) modified demineralized bone matrix (DBM) which provided a more effective osteogenesis and regeneration for critically-sized femur defects in goats using the selective cell retention (SCR) strategy. METHODS: RADA16-I peptide was used to modify DBM and formed a composite scaffold (SAP/DBM). The morphological change and dynamic expression of osteogenic genes of mesenchymal stem cells (MSCs) derived from marrow in SAP/DBM was observed. The cells and factors in bone marrow were enriched into SAP/DBM by technology of selective cells retension (SCR). The construct was transplanted into 20-mm femur defects in goats and their osteogenesis was evaluated. RESULTS: The SAP/DBM scaffold formed a three-dimensional interweaving nanofiber in pores of DBM. MSCs exhibited better morphology in SAP/DBM than that in only DBM, and the levels of expression of ALP ,OCN and Runx2 gene in SAP/DBM samples was significantly higher than that of DBM at 14 days in vitro (P < 0.05). Compared with marrow-enriched DBM, the volume of newly formed bone from marrow-enriched SAP/DBM is higher in goats (P < 0.05). CONCLUSION: Our study may not only have a significant impact on the construction method of tissue engineering but also provide a viable, simple and effective method for clinical bone construction.
Authors: Michael E Davis; J P Michael Motion; Daria A Narmoneva; Tomosaburo Takahashi; Daihiko Hakuno; Roger D Kamm; Shuguang Zhang; Richard T Lee Journal: Circulation Date: 2005-02-01 Impact factor: 29.690
Authors: Johannes C Reichert; Martin E Wullschleger; Amaia Cipitria; Jasmin Lienau; Tan K Cheng; Michael A Schütz; Georg N Duda; Ulrich Nöth; Jochen Eulert; Dietmar W Hutmacher Journal: Int Orthop Date: 2010-12-07 Impact factor: 3.075
Authors: Philip D Tatman; Ethan G Muhonen; Sean T Wickers; Albert O Gee; Eung-Sam Kim; Deok-Ho Kim Journal: Biomater Sci Date: 2016-02-15 Impact factor: 6.843
Authors: Ippokratis Pountos; Michalis Panteli; Anastasios Lampropoulos; Elena Jones; Giorgio Maria Calori; Peter V Giannoudis Journal: BMC Med Date: 2016-07-11 Impact factor: 8.775