Rong Tian1, DongFeng Pan2. 1. Department of Nuclear Medicine, Sichuan University, 37 Guoxuexiang, Chengdu, China 610041. 2. Radiology Department, University of Virginia, P.O. Box 800170, Charlottesville, VA 22908 USA.
Abstract
AIM: The aim of this article is to present the results of an imaging study of myocardial apoptosis induced by ischemia/reperfusion injury. METHODS: Twenty nude mice were randomly divided into an experimental group (10 mice) and control group (10 mice). In the experimental group, myocardial apoptosis was induced by ligation of the left anterior descending coronary artery (LAD) for 30 min. This was followed by reperfusion for 90 min. In the control group, the heart was exposed for the same length of time as in the experimental group. Cy5.5-annexin V (25 μg) was injected into both sets of mice after the onset of reperfusion. At 90 min post-injection, the mice were imaged. The region of interest (ROI) was obtained, and the fluorescence intensity of the ROI was quantified. The animals were sacrificed, and myocardial apoptosis was assayed by TUNEL assay. RESULTS: Fluorescence intensity in the ischemia/reperfusion hearts was significantly higher than that in the control group (P < 0.05). In the TUNEL assay, more apoptotic cells were observed in the experimental group than in the control group, correlating with imaging results. CONCLUSION: Fluorescence imaging of Cy5.5-annexin V in a mouse model of myocardial ischemia/reperfusion can be used in vivo as a noninvasive means of detecting ischemia/reperfusion-induced apoptotic cells in the heart.
AIM: The aim of this article is to present the results of an imaging study of myocardial apoptosis induced by ischemia/reperfusion injury. METHODS: Twenty nude mice were randomly divided into an experimental group (10 mice) and control group (10 mice). In the experimental group, myocardial apoptosis was induced by ligation of the left anterior descending coronary artery (LAD) for 30 min. This was followed by reperfusion for 90 min. In the control group, the heart was exposed for the same length of time as in the experimental group. Cy5.5-annexin V (25 μg) was injected into both sets of mice after the onset of reperfusion. At 90 min post-injection, the mice were imaged. The region of interest (ROI) was obtained, and the fluorescence intensity of the ROI was quantified. The animals were sacrificed, and myocardial apoptosis was assayed by TUNEL assay. RESULTS: Fluorescence intensity in the ischemia/reperfusion hearts was significantly higher than that in the control group (P < 0.05). In the TUNEL assay, more apoptotic cells were observed in the experimental group than in the control group, correlating with imaging results. CONCLUSION: Fluorescence imaging of Cy5.5-annexin V in a mouse model of myocardial ischemia/reperfusion can be used in vivo as a noninvasive means of detecting ischemia/reperfusion-induced apoptotic cells in the heart.
Authors: D J Yang; A Azhdarinia; P Wu; D F Yu; W Tansey; S K Kalimi; E E Kim; D A Podoloff Journal: Cancer Biother Radiopharm Date: 2001-02 Impact factor: 3.099
Authors: F G Blankenberg; P D Katsikis; J F Tait; R E Davis; L Naumovski; K Ohtsuki; S Kopiwoda; M J Abrams; H W Strauss Journal: J Nucl Med Date: 1999-01 Impact factor: 10.057
Authors: F G Blankenberg; P D Katsikis; J F Tait; R E Davis; L Naumovski; K Ohtsuki; S Kopiwoda; M J Abrams; M Darkes; R C Robbins; H T Maecker; H W Strauss Journal: Proc Natl Acad Sci U S A Date: 1998-05-26 Impact factor: 11.205
Authors: Hendrikus H Boersma; Bas L J H Kietselaer; Leo M L Stolk; Abdelkader Bennaghmouch; Leonard Hofstra; Jagat Narula; Guido A K Heidendal; Chris P M Reutelingsperger Journal: J Nucl Med Date: 2005-12 Impact factor: 10.057
Authors: B Freude; T N Masters; F Robicsek; A Fokin; S Kostin; R Zimmermann; C Ullmann; S Lorenz-Meyer; J Schaper Journal: J Mol Cell Cardiol Date: 2000-02 Impact factor: 5.000
Authors: Yan Hu; Guobing Liu; He Zhang; Yanli Li; Brian D Gray; Koon Y Pak; Hak Soo Choi; Dengfeng Cheng; Hongcheng Shi Journal: Mol Imaging Biol Date: 2018-04 Impact factor: 3.488
Authors: Jonathan W Hardy; Zoia Levashova; Tobi L Schmidt; Christopher H Contag; Francis G Blankenberg Journal: Mol Imaging Biol Date: 2015-06 Impact factor: 3.488