Hyun-Woo Kwon1, Ho-Young Lee2, Seog Gyun Kim1, Won Kim3, Yong Jin Jung3, Keon Wook Kang4, June-Key Chung4, Myung Chul Lee4, Dong Soo Lee4. 1. Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea. 2. Department of Nuclear Medicine, Seoul National University Boramae Medical Center, 39, Boramae-Gil, Dongjak-Gu, Seoul, 156-707 Korea ; Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea. 3. Department of Internal Medicine, Seoul National University Boramae Medical Center, Seoul, Korea. 4. Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea ; Institute of Radiation Medicine, Medical Research Center, Seoul National University, Seoul, Korea.
Abstract
PURPOSE: Measurement of serum hepatitis B virus surface antigen (HBsAg) levels is important for the management of chronic hepatitis B patients in terms of monitoring response to antiviral therapy. This study aimed to evaluate the diagnostic performance of a new diagnostic kit, which quantitatively measures serum HBsAg level using an immunoradiometric assay (IRMA)-based method. Measurements were compared with those obtained using a chemiluminescent microparticle immunoassay (CMIA)-based method. METHODS: The blood samples of 96 patients with chronic hepatitis B were used in this study. Copy numbers of serum hepatitis B virus (HBV) DNA were determined in 23 of these samples. The correlation between and the concordance of IRMA and CMIA results were determined using Pearson's correlation coefficients. P values of 0.05 were considered to be statistically significant throughout. RESULTS: Laboratory diagnoses based on IRMA were wholly in agreement with those based on CMIA. Furthermore, serum HBsAg levels by IRMA were found to be highly correlated with those determined by CMIA (correlation coefficient R (2) = 0.838, p < 0.001). Serum HBsAg level and serum HBV DNA copies were found to be linearly related by both methods (R (2) = 0.067, p = 0.316 by IRMA, and R (2) = 0.101, p = 0.215 by CMIA). CONCLUSION: The diagnostic performance of the investigated IRMA method of determining HBsAg levels was found to be comparable with that of a CMIA-based method in chronic hepatitis B patients.
PURPOSE: Measurement of serum hepatitis B virus surface antigen (HBsAg) levels is important for the management of chronic hepatitis Bpatients in terms of monitoring response to antiviral therapy. This study aimed to evaluate the diagnostic performance of a new diagnostic kit, which quantitatively measures serum HBsAg level using an immunoradiometric assay (IRMA)-based method. Measurements were compared with those obtained using a chemiluminescent microparticle immunoassay (CMIA)-based method. METHODS: The blood samples of 96 patients with chronic hepatitis B were used in this study. Copy numbers of serum hepatitis B virus (HBV) DNA were determined in 23 of these samples. The correlation between and the concordance of IRMA and CMIA results were determined using Pearson's correlation coefficients. P values of 0.05 were considered to be statistically significant throughout. RESULTS: Laboratory diagnoses based on IRMA were wholly in agreement with those based on CMIA. Furthermore, serum HBsAg levels by IRMA were found to be highly correlated with those determined by CMIA (correlation coefficient R (2) = 0.838, p < 0.001). Serum HBsAg level and serum HBV DNA copies were found to be linearly related by both methods (R (2) = 0.067, p = 0.316 by IRMA, and R (2) = 0.101, p = 0.215 by CMIA). CONCLUSION: The diagnostic performance of the investigated IRMA method of determining HBsAg levels was found to be comparable with that of a CMIA-based method in chronic hepatitis Bpatients.
Authors: G Fattovich; G Giustina; J Sanchez-Tapias; C Quero; A Mas; P G Olivotto; A Solinas; P Almasio; S Hadziyannis; F Degos; M C de Moura; K Krogsgaard; M Pantalena; G Realdi; R Corrocher; S W Schalm Journal: Am J Gastroenterol Date: 1998-06 Impact factor: 10.864
Authors: Kwang Hyun Chung; Won Kim; Byeong Gwan Kim; Ho-Young Lee; Eunhyo Jin; Yuri Cho; Ji Yeon Seo; Hwi Young Kim; Yong Jin Jung; Ji Won Kim; Ji Bong Jeong; Kook Lae Lee Journal: Gut Liver Date: 2015-09-23 Impact factor: 4.519
Authors: Goh Eun Chung; Ju Yeon Kim; Hyunjae Shin; Ji Hoon Hong; Moon Haeng Hur; Heejin Cho; Min Kyung Park; Na Ryung Choi; Jihye Kim; Yun Bin Lee; Eun Ju Cho; Su Jong Yu; Yoon Jun Kim; Jung-Hwan Yoon; Jeong-Hoon Lee Journal: Diagnostics (Basel) Date: 2022-07-20