Wei Zhou1, Yangyang Xu2, Xingang Li2, Ge Gao3, Zheng Jiang2, Zhenyu Shao4. 1. Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Jinan, Shandong, China. 2. Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan, Shandong, China Brain Science Research Institute, Shandong University, Jinan, Shandong, China. 3. Affiliated Hospital, Shandong Academy of Medical Sciences, Jinan, Shandong, China. 4. Department of Radiotherapy, Cancer Centre, Qilu Hospital, Shandong University, Jinan, Shandong, China drzhenyushao@hotmail.com.
Abstract
OBJECTIVE: To investigate levels of chemokine (C-X-C motif) receptor 4 (cxcr4) mRNA and protein in X-irradiated glioma cells. METHODS: Murine malignant glioma GL261 cells transfected with hypoxia-inducible factor (HIF)-1α miRNA or control miRNA were irradiated with X-radiation. Cxcr4 mRNA and protein were analysed using real-time reverse transcription-polymerase chain reaction and Western blot, respectively. RESULTS: Levels of cxcr4 protein in GL261 cells increased in a radiation dose-dependent manner 48 h after 0, 5, 10 and 15 Gy X-irradiation. Irradiation of both HIF-1α knockdown cells and control cells resulted in a significant increase in cxcr4 mRNA levels, compared with nonirradiated cells, at 24 h after 5 Gy X-irradiation. CONCLUSION: Irradiation enhances expression of cxcr4 in glioma cells via a HIF-1α-independent pathway.
OBJECTIVE: To investigate levels of chemokine (C-X-C motif) receptor 4 (cxcr4) mRNA and protein in X-irradiated glioma cells. METHODS:Murine malignant glioma GL261 cells transfected with hypoxia-inducible factor (HIF)-1α miRNA or control miRNA were irradiated with X-radiation. Cxcr4 mRNA and protein were analysed using real-time reverse transcription-polymerase chain reaction and Western blot, respectively. RESULTS: Levels of cxcr4 protein in GL261 cells increased in a radiation dose-dependent manner 48 h after 0, 5, 10 and 15 Gy X-irradiation. Irradiation of both HIF-1α knockdown cells and control cells resulted in a significant increase in cxcr4 mRNA levels, compared with nonirradiated cells, at 24 h after 5 Gy X-irradiation. CONCLUSION: Irradiation enhances expression of cxcr4 in glioma cells via a HIF-1α-independent pathway.