OBJECTIVE: To establish an accurate anaesthetic dose for chemical restraint of African mole-rats using ketamine and xylazine. STUDY DESIGN: Prospective nonrandomized laboratory study. ANIMALS: Sixteen adult Ansell's mole-rats (Fukomys anselli) and eight giant mole-rats (F. mechowii). METHODS: Fukomys anselli of different ages, sexes and reproductive status were systematically anaesthetized starting with an intramuscular injection of ketamine (2.5 mg kg(-1) ) and increasing the doses in steps of 0.5 mg kg(-1) until loss of the righting reflex (LRR) was observed. Xylazine was added to a constant dose of ketamine, starting at 0.5 mg kg(-1) that was increased by 0.5 mg kg(-1) in further trials. Once an effective combination was established and evaluated in F. anselli, it was also tested in F. mechowii. Heart and respiratory rates and rectal temperatures were measured during anaesthesia. anova for repeated measures and Student's t-test were used to compare means. RESULTS: Chemical restraint was accomplished at a dose of 6 mg kg(-1) ketamine combined with 2.5 mg kg(-1) xylazine. LRR lasted on average mean 56 ± SD 19 minutes (F. anselli) and 140 ± 41 minutes (F. mechowii). Loss of pedal withdrawal reflex (LPR) lasted for 20 ± 15 minutes (F. anselli) and for 29 ± 2 minutes (F. mechowii), respectively. All animals recovered satisfactorily. Heart and respiratory rates were stable during anaesthesia, but rectal temperature fell significantly in F. mechowii after losing the righting reflex (LRR) from T1 (32.6 ± 0.6 °C) to T3 (30.4 ± 0.9 °C). CONCLUSIONS AND CLINICAL RELEVANCE: African mole-rats (Bathyergidae) live in closed burrow systems under particular conditions (hypercapnia, hypoxia, stable temperature, humidity, darkness) and show several physiological adaptations. Injectable anaesthetics in the dose rates used in other rodents are not appropriate for use in these subterranean species. Here, a reliable protocol for chemical restraint is provided.
OBJECTIVE: To establish an accurate anaesthetic dose for chemical restraint of African mole-rats using ketamine and xylazine. STUDY DESIGN: Prospective nonrandomized laboratory study. ANIMALS: Sixteen adult Ansell's mole-rats (Fukomys anselli) and eight giant mole-rats (F. mechowii). METHODS:Fukomys anselli of different ages, sexes and reproductive status were systematically anaesthetized starting with an intramuscular injection of ketamine (2.5 mg kg(-1) ) and increasing the doses in steps of 0.5 mg kg(-1) until loss of the righting reflex (LRR) was observed. Xylazine was added to a constant dose of ketamine, starting at 0.5 mg kg(-1) that was increased by 0.5 mg kg(-1) in further trials. Once an effective combination was established and evaluated in F. anselli, it was also tested in F. mechowii. Heart and respiratory rates and rectal temperatures were measured during anaesthesia. anova for repeated measures and Student's t-test were used to compare means. RESULTS: Chemical restraint was accomplished at a dose of 6 mg kg(-1) ketamine combined with 2.5 mg kg(-1) xylazine. LRR lasted on average mean 56 ± SD 19 minutes (F. anselli) and 140 ± 41 minutes (F. mechowii). Loss of pedal withdrawal reflex (LPR) lasted for 20 ± 15 minutes (F. anselli) and for 29 ± 2 minutes (F. mechowii), respectively. All animals recovered satisfactorily. Heart and respiratory rates were stable during anaesthesia, but rectal temperature fell significantly in F. mechowii after losing the righting reflex (LRR) from T1 (32.6 ± 0.6 °C) to T3 (30.4 ± 0.9 °C). CONCLUSIONS AND CLINICAL RELEVANCE: African mole-rats (Bathyergidae) live in closed burrow systems under particular conditions (hypercapnia, hypoxia, stable temperature, humidity, darkness) and show several physiological adaptations. Injectable anaesthetics in the dose rates used in other rodents are not appropriate for use in these subterranean species. Here, a reliable protocol for chemical restraint is provided.
Authors: Steve Hoffmann; Karol Szafranski; Philip Dammann; Arne Sahm; Matthias Platzer; Philipp Koch; Yoshiyuki Henning; Martin Bens; Marco Groth; Hynek Burda; Sabine Begall; Saskia Ting; Moritz Goetz; Paul Van Daele; Magdalena Staniszewska; Jasmin Mona Klose; Pedro Fragoso Costa Journal: Elife Date: 2021-03-16 Impact factor: 8.140
Authors: Yoshiyuki Henning; Christiane Vole; Sabine Begall; Martin Bens; Martina Broecker-Preuss; Arne Sahm; Karol Szafranski; Hynek Burda; Philip Dammann Journal: PLoS One Date: 2014-11-19 Impact factor: 3.240