F Catal1, E Mete2, C Tayman2, E Topal3, A Albayrak4, H Sert5. 1. Department of Pediatric Allergy and Asthma, Fatih University Faculty of Medicine, Ankara, Turkey; Department of Anesthesia, Fatih University Faculty of Medicine, Ankara, Turkey. 2. Department of Pediatric Allergy and Asthma, Fatih University Faculty of Medicine, Ankara, Turkey. 3. Department of Pediatric Allergy and Asthma, Gazi University Faculty of Medicine, Ankara, Turkey. Electronic address: erdemtopal44@gmail.com. 4. Department of Pathology, Ankara Numune Education and Research Hospital, Ankara, Turkey. 5. Department of Anesthesia, Fatih University Faculty of Medicine, Ankara, Turkey.
Abstract
BACKGROUND: A few experimental studies related to asthma have unveiled the beneficial effects of TNF alpha blocking agents on the airway histology, cytokine levels in bronchoalveolar lavage and bronchial hyper-responsiveness. In the current study, we aimed to assess the effect of adalimumab on the inflammation and histology of asthma in a murine model. METHOD: Twelve-week-old BALB/c (H-2d/d) female rats (n=18) were allocated into three groups, including (group I) control (phosphate-buffered saline was implemented), (group II) asthma induced with OVA (n=6), and (group III) asthma induced with OVA+treated with adalimumab (n=6). Rats were executed on the 28th day of the study. The lung samples were fixed in 10% neutral buffered formalin. Lung parenchyma, alveolus, peribronchial and perivascular inflammation were assessed. Lung pathological scoring was performed. RESULT: Severity of lung damage was found to be reduced significantly in the asthma induced with OVA+treated with adalimumab group. When compared with the untreated group, adalimumab significantly reduced the inflammatory cells around the bronchi and bronchioles, and reduced inflammation of the alveolar wall and alveolar wall thickness as well (median score=1, p=0.52). Peribronchial smooth muscle hypertrophy and oedema were significantly reduced after adalimumab administration. CONCLUSION: Adalimumab (a human monoclonal anti-TNF alpha antibody) therapy significantly reduced the severity of lung damage by decreasing cellular infiltration and improvement on the lung histology in a murine model of acute asthma.
BACKGROUND: A few experimental studies related to asthma have unveiled the beneficial effects of TNF alpha blocking agents on the airway histology, cytokine levels in bronchoalveolar lavage and bronchial hyper-responsiveness. In the current study, we aimed to assess the effect of adalimumab on the inflammation and histology of asthma in a murine model. METHOD: Twelve-week-old BALB/c (H-2d/d) female rats (n=18) were allocated into three groups, including (group I) control (phosphate-buffered saline was implemented), (group II) asthma induced with OVA (n=6), and (group III) asthma induced with OVA+treated with adalimumab (n=6). Rats were executed on the 28th day of the study. The lung samples were fixed in 10% neutral buffered formalin. Lung parenchyma, alveolus, peribronchial and perivascular inflammation were assessed. Lung pathological scoring was performed. RESULT: Severity of lung damage was found to be reduced significantly in the asthma induced with OVA+treated with adalimumab group. When compared with the untreated group, adalimumab significantly reduced the inflammatory cells around the bronchi and bronchioles, and reduced inflammation of the alveolar wall and alveolar wall thickness as well (median score=1, p=0.52). Peribronchial smooth muscle hypertrophy and oedema were significantly reduced after adalimumab administration. CONCLUSION:Adalimumab (a human monoclonal anti-TNF alpha antibody) therapy significantly reduced the severity of lung damage by decreasing cellular infiltration and improvement on the lung histology in a murine model of acute asthma.
Authors: Rama Malaviya; Alyssa Bellomo; Elena Abramova; Claire R Croutch; Julie Roseman; Rick Tuttle; Eric Peters; Robert P Casillas; Vasanthi R Sunil; Jeffrey D Laskin; Debra L Laskin Journal: Toxicol Appl Pharmacol Date: 2021-08-11 Impact factor: 4.460
Authors: Nathalia M Pinheiro; Claudia J C P Miranda; Adenir Perini; Niels O S Câmara; Soraia K P Costa; Maria Isabel C Alonso-Vale; Luciana C Caperuto; Iolanda F L C Tibério; Marco Antônio M Prado; Mílton A Martins; Vânia F Prado; Carla M Prado Journal: PLoS One Date: 2015-03-27 Impact factor: 3.240