PURPOSE: Although many studies have been performed to evaluate whether or not apolipoprotein E gene (APOE) polymorphisms are differentially associated with bone mineral density (BMD) and fractures, the results have been conflicting. This large-scale study was performed to investigate whether a relationship exists between APOE polymorphisms and risk of fracture. METHODS: A hospital-based case-control study was conducted in 3,000 patients with fractures and 3,000 age- and gender-matched healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was applied to assess the APOE gene polymorphisms. RESULTS: Patients with fractures had a significantly higher frequency of APOE E2/E2 genotype [odds ratio (OR) = 2.02, 95% confidence interval (CI) = 1.30, 3.14; P = 0.002] than healthy controls. When stratifying by fracture type, it was found that patients with vertebral fractures had a significantly higher frequency of APOE E2/E2 genotype (OR = 2.86, 95% CI = 1.73, 4.73; P < 0.001). No significant differences were found in nonvertebral (hip or wrist or other) fractures. CONCLUSIONS: Our study suggests that APOE E2/E2 genotype is a potential genetic risk factor for vertebral fractures in humans.
PURPOSE: Although many studies have been performed to evaluate whether or not apolipoprotein E gene (APOE) polymorphisms are differentially associated with bone mineral density (BMD) and fractures, the results have been conflicting. This large-scale study was performed to investigate whether a relationship exists between APOE polymorphisms and risk of fracture. METHODS: A hospital-based case-control study was conducted in 3,000 patients with fractures and 3,000 age- and gender-matched healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay was applied to assess the APOE gene polymorphisms. RESULTS:Patients with fractures had a significantly higher frequency of APOE E2/E2 genotype [odds ratio (OR) = 2.02, 95% confidence interval (CI) = 1.30, 3.14; P = 0.002] than healthy controls. When stratifying by fracture type, it was found that patients with vertebral fractures had a significantly higher frequency of APOE E2/E2 genotype (OR = 2.86, 95% CI = 1.73, 4.73; P < 0.001). No significant differences were found in nonvertebral (hip or wrist or other) fractures. CONCLUSIONS: Our study suggests that APOE E2/E2 genotype is a potential genetic risk factor for vertebral fractures in humans.
Authors: John P A Ioannidis; Ioanna Stavrou; Thomas A Trikalinos; Christos Zois; Maria Luisa Brandi; Luigi Gennari; Omar Albagha; Stuart H Ralston; Agathocles Tsatsoulis Journal: J Bone Miner Res Date: 2002-11 Impact factor: 6.741
Authors: Mariette W C J Schoofs; Marjolein van der Klift; Albert Hofman; Cornelia M van Duijn; Bruno H Ch Stricker; Huibert A P Pols; André G Uitterlinden Journal: J Bone Miner Res Date: 2004-06-21 Impact factor: 6.741
Authors: Rong Huang; Xiaohua Zong; Puviindran Nadesan; Janet L Huebner; Virginia B Kraus; James P White; Phillip J White; Gurpreet S Baht Journal: JCI Insight Date: 2019-09-19
Authors: Candice L Downey; Adam Young; Emily F Burton; Simon M Graham; Robert J Macfarlane; Eva-Maria Tsapakis; Eleftherios Tsiridis Journal: World J Orthop Date: 2017-05-18