Wellington J Rody1, L Shannon Holliday2, Kevin P McHugh3, Shannon M Wallet3, Victor Spicer4, Oleg Krokhin5. 1. Assistant professor, Department of Orthodontics, College of Dentistry, University of Florida, Gainesville, Fla. Electronic address: wrody@dental.ufl.edu. 2. Associate professor, Department of Orthodontics, College of Dentistry, and Department of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, Fla. 3. Associate professor, Department of Periodontology, College of Dentistry, University of Florida, Gainesville, Fla. 4. Bioinformatics specialist, Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada. 5. Assistant professor, Department of Internal Medicine, University of Manitoba; senior scientist, Manitoba Center for Proteomics and Systems Biology, Winnipeg Manitoba, Canada.
Abstract
INTRODUCTION: In this study, we used liquid chromatography-mass spectrometry (LC-MS) to investigate the differences in the composition of gingival crevicular fluid between resorbing deciduous molars and nonresorbing permanent teeth. The main goal was to identify novel biomarkers associated with root resorption. METHODS: Eleven children (4 boys, 7 girls) in the mixed dentition were selected to participate in this split-mouth design study, in which a deciduous second molar with radiographic evidence of root resorption served as the experimental site, and the permanent first molar on the contralateral quadrant was the control site. Gingival crevicular fluid was collected using absorbing strips. A total of 22 samples (11 root resorption, 11 control) were each analyzed with 1-dimensional LC-MS. The remaining samples were then pooled across the 11 patients and analyzed by 2-dimensional LC-MS. The output files were converted to mascot generic format, which can be used to perform protein identification with conventional search engines. RESULTS: The 2-dimensional LC-MS protocol was able to identify 2789 and 2421 proteins in the control and resorption pooled samples, respectively. In this population, we detected significantly upregulated and downregulated proteins in the teeth with root resorption. Interestingly, many of these proteins are characteristically found in exosomes. CONCLUSIONS: We identified novel proteins that might prove to be useful biomarkers of root resorption, individually or as part of a panel.
INTRODUCTION: In this study, we used liquid chromatography-mass spectrometry (LC-MS) to investigate the differences in the composition of gingival crevicular fluid between resorbing deciduous molars and nonresorbing permanent teeth. The main goal was to identify novel biomarkers associated with root resorption. METHODS: Eleven children (4 boys, 7 girls) in the mixed dentition were selected to participate in this split-mouth design study, in which a deciduous second molar with radiographic evidence of root resorption served as the experimental site, and the permanent first molar on the contralateral quadrant was the control site. Gingival crevicular fluid was collected using absorbing strips. A total of 22 samples (11 root resorption, 11 control) were each analyzed with 1-dimensional LC-MS. The remaining samples were then pooled across the 11 patients and analyzed by 2-dimensional LC-MS. The output files were converted to mascot generic format, which can be used to perform protein identification with conventional search engines. RESULTS: The 2-dimensional LC-MS protocol was able to identify 2789 and 2421 proteins in the control and resorption pooled samples, respectively. In this population, we detected significantly upregulated and downregulated proteins in the teeth with root resorption. Interestingly, many of these proteins are characteristically found in exosomes. CONCLUSIONS: We identified novel proteins that might prove to be useful biomarkers of root resorption, individually or as part of a panel.
Authors: N Huynh; L VonMoss; D Smith; I Rahman; M F Felemban; J Zuo; W J Rody; K P McHugh; L S Holliday Journal: J Dent Res Date: 2016-02-23 Impact factor: 6.116
Authors: Wellington J Rody; Casey A Chamberlain; Alyssa K Emory-Carter; Kevin P McHugh; Shannon M Wallet; Victor Spicer; Oleg Krokhin; L Shannon Holliday Journal: PLoS One Date: 2019-07-10 Impact factor: 3.240