Toshio Hisatomi1, Shoji Notomi2, Takashi Tachibana3, Yukio Sassa3, Yasuhiro Ikeda3, Takao Nakamura3, Akifumi Ueno3, Hiroshi Enaida3, Toshinori Murata3, Taiji Sakamoto4, Tatsuro Ishibashi3. 1. Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Clinical Research Institute, Kyushu Medical Centre, Fukuoka, Japan. Electronic address: hisatomi@med.kyushu-u.ac.jp. 2. Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Clinical Research Institute, Kyushu Medical Centre, Fukuoka, Japan. 3. Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. 4. Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Department of Ophthalmology, Kagoshima University School of Medicine, Kagoshima, Japan.
Abstract
PURPOSE: To investigate long-term ultrastructural changes in the retina after internal limiting membrane (ILM) peeling through the examination of morphologic changes 3 years after vitrectomy in cynomolgus monkeys. DESIGN: Laboratory investigation. METHODS: Pars plana vitrectomy was performed, followed by ILM peeling, in 2 primate eyes. Ultrastructural changes were investigated using light microscopy and transmission and scanning electron microscopy 3 years after ILM peeling. RESULTS: The remaining posterior vitreous and ILM-peeled areas were clearly recognized after the long-term follow-up. The exposed Müller cell processes were partially damaged, while regenerative spindle-shaped Müller cell processes developed, covering most of the retina. Notably, the nerve fiber layer was found to be uncovered and exposed to the vitreous space owing to misdirection of glial wound healing in some parts. In these areas, glial wound healing occurred beneath the nerve fiber layer. Although the glial cells covered the damaged areas, there was no apparent ILM regeneration in the shape of a continuous flat sheet, with the exception of accumulated deposits of basement membrane materials. CONCLUSIONS: Although the retinal structures were well preserved after ILM peeling, ILM peeling resulted in mild damage to the vitreoretinal interface, which was not completely restored even after 3 years. The multilinear shape of the exposed nerve fiber may explain the previously reported dissociated optic nerve fiber layer appearance. The glial cells produced basement membrane materials around their processes, although they did not restore the ILM as a flat sheet.
PURPOSE: To investigate long-term ultrastructural changes in the retina after internal limiting membrane (ILM) peeling through the examination of morphologic changes 3 years after vitrectomy in cynomolgus monkeys. DESIGN: Laboratory investigation. METHODS: Pars plana vitrectomy was performed, followed by ILM peeling, in 2 primate eyes. Ultrastructural changes were investigated using light microscopy and transmission and scanning electron microscopy 3 years after ILM peeling. RESULTS: The remaining posterior vitreous and ILM-peeled areas were clearly recognized after the long-term follow-up. The exposed Müller cell processes were partially damaged, while regenerative spindle-shaped Müller cell processes developed, covering most of the retina. Notably, the nerve fiber layer was found to be uncovered and exposed to the vitreous space owing to misdirection of glial wound healing in some parts. In these areas, glial wound healing occurred beneath the nerve fiber layer. Although the glial cells covered the damaged areas, there was no apparent ILM regeneration in the shape of a continuous flat sheet, with the exception of accumulated deposits of basement membrane materials. CONCLUSIONS: Although the retinal structures were well preserved after ILM peeling, ILM peeling resulted in mild damage to the vitreoretinal interface, which was not completely restored even after 3 years. The multilinear shape of the exposed nerve fiber may explain the previously reported dissociated optic nerve fiber layer appearance. The glial cells produced basement membrane materials around their processes, although they did not restore the ILM as a flat sheet.
Authors: Justis P Ehlers; Jaehong Han; Daniel Petkovsek; Peter K Kaiser; Rishi P Singh; Sunil K Srivastava Journal: Invest Ophthalmol Vis Sci Date: 2015-11 Impact factor: 4.799
Authors: Sanjar Batirovich Madrakhimov; Jin Young Yang; Dong Hyuck Ahn; Jung Woo Han; Tae Ho Ha; Tae Kwann Park Journal: Mol Ther Methods Clin Dev Date: 2020-03-30 Impact factor: 6.698