| Literature DB >> 24878069 |
Amina Yssouf1, Cristina Socolovschi1, Hamza Leulmi1, Tahar Kernif2, Idir Bitam3, Gilles Audoly1, Lionel Almeras1, Didier Raoult4, Philippe Parola5.
Abstract
In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas.Entities:
Keywords: Fleas; Identification; MALDI-TOF-mass spectrometry
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Year: 2014 PMID： 24878069 DOI： 10.1016/j.cimid.2014.05.002
Source DB: PubMed Journal: Comp Immunol Microbiol Infect Dis ISSN： 0147-9571 Impact factor: 2.268