Li Hao1, Xiao-Guang Wang1, Jian-Ding Cheng1, Sheng-Zhong You1, Su-Hua Ma1, Xia Zhong1, Li Quan2, Bin Luo3. 1. Faculty of Forensic Medicine, Sun Yat-Sen University Zhongshan School of Medicine, No. 74, Zhongshan 2 Road, Guangzhou 510080, Guangdong province, P.R. China. 2. Faculty of Forensic Medicine, Sun Yat-Sen University Zhongshan School of Medicine, No. 74, Zhongshan 2 Road, Guangzhou 510080, Guangdong province, P.R. China; Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 1-4-3, Abeno, Osaka 545-8585, Japan. Electronic address: quanli2@mail.sysu.edu.cn. 3. Faculty of Forensic Medicine, Sun Yat-Sen University Zhongshan School of Medicine, No. 74, Zhongshan 2 Road, Guangzhou 510080, Guangdong province, P.R. China. Electronic address: luobin@mail.sysu.edu.cn.
Abstract
BACKGROUND: Previous studies have reported important roles of endothelin-1 (ET-1) and angiotensin II (Ang II) in the pathogenesis of atherosclerosis. However, the expression of these two proteins and the underlying mechanisms in human atherosclerotic coronary arteries are largely unknown. METHODS: We examined the expression of ET-1 and Ang II in pericardial fluid and coronary arteries from 25 individuals (n = 25) using enzyme-linked immuno sorbent assay (ELISA) and immunohistochemistry. Twelve patients died from acute coronary syndrome were classified as atherosclerotic plaque group (AP group) (n = 12), while 13 patients died from other causes were classified as non-AP group (n = 13). Meanwhile, we performed reverse transcription-polymerase chain reaction (RT-PCR) to measure the expression of six microRNAs targeting ET-1 in formalin-fixed, paraffin-embedded coronary arteries. RESULTS: Our data showed that ET-1 was significantly higher in both pericardial fluid and coronary arteries from AP group. However, Ang II showed no significant difference in pericardial fluid between the two groups, while it was even significantly lower in coronary arteries from AP group. Besides, miR-125a-5p, miR-155, and miR-199a/b-3p, which suppressed the expression of ET-1, were down-regulated in the coronary arteries from AP group. CONCLUSION: The up-regulation of ET-1, regulated by miR-125a-5p, miR-155, and miR-199a/b-3p, indicated that ET-1 played an important role in human coronary atherosclerosis. SUMMARY: We focused on the human coronary arteries with atherosclerotic plaques. The expression of ET-1, as well as its upstream miRNAs, was determined. Unlike any of previous study regarding miRNAs expression, we could exclude the discrepancy of artery-bed-specific miRNA expression. Besides, our data indicated, to some degree, that ET-1 might play a more vital role than Ang II in coronary atherosclerosis.
BACKGROUND: Previous studies have reported important roles of endothelin-1 (ET-1) and angiotensin II (Ang II) in the pathogenesis of atherosclerosis. However, the expression of these two proteins and the underlying mechanisms in humanatherosclerotic coronary arteries are largely unknown. METHODS: We examined the expression of ET-1 and Ang II in pericardial fluid and coronary arteries from 25 individuals (n = 25) using enzyme-linked immuno sorbent assay (ELISA) and immunohistochemistry. Twelve patients died from acute coronary syndrome were classified as atherosclerotic plaque group (AP group) (n = 12), while 13 patients died from other causes were classified as non-AP group (n = 13). Meanwhile, we performed reverse transcription-polymerase chain reaction (RT-PCR) to measure the expression of six microRNAs targeting ET-1 in formalin-fixed, paraffin-embedded coronary arteries. RESULTS: Our data showed that ET-1 was significantly higher in both pericardial fluid and coronary arteries from AP group. However, Ang II showed no significant difference in pericardial fluid between the two groups, while it was even significantly lower in coronary arteries from AP group. Besides, miR-125a-5p, miR-155, and miR-199a/b-3p, which suppressed the expression of ET-1, were down-regulated in the coronary arteries from AP group. CONCLUSION: The up-regulation of ET-1, regulated by miR-125a-5p, miR-155, and miR-199a/b-3p, indicated that ET-1 played an important role in humancoronary atherosclerosis. SUMMARY: We focused on the human coronary arteries with atherosclerotic plaques. The expression of ET-1, as well as its upstream miRNAs, was determined. Unlike any of previous study regarding miRNAs expression, we could exclude the discrepancy of artery-bed-specific miRNA expression. Besides, our data indicated, to some degree, that ET-1 might play a more vital role than Ang II in coronary atherosclerosis.
Authors: Jamie G Hijmans; Ma'ayan Levy; Vinicius Garcia; Grace M Lincenberg; Kyle J Diehl; Jared J Greiner; Brian L Stauffer; Christopher A DeSouza Journal: Exp Physiol Date: 2019-04-30 Impact factor: 2.969
Authors: Bernd Frank; Liana Ariza; Heidrun Lamparter; Vera Grossmann; Jürgen H Prochaska; Alexander Ullmann; Florentina Kindler; Gerhard Weisser; Ulrich Walter; Karl J Lackner; Christine Espinola-Klein; Thomas Münzel; Stavros V Konstantinides; Philipp S Wild Journal: BMJ Open Date: 2015-07-01 Impact factor: 2.692