Zhi-Kuan Xia1, Qiu-Hua Ma2, Shu-Yi Li2, De-Quan Zhang2, Lin Cong2, Yan-Li Tian2, Rong-Ya Yang3. 1. Department of Dermatology, General Hospital of Beijing Military Command of PLA, Nanmencang, Dongcheng District, Beijing, PR China; Third Military Medical University, Gaotanyanzhengjie, Shapingba District, Chongqing, PR China. 2. Department of Dermatology, General Hospital of Beijing Military Command of PLA, Nanmencang, Dongcheng District, Beijing, PR China. 3. Department of Dermatology, General Hospital of Beijing Military Command of PLA, Nanmencang, Dongcheng District, Beijing, PR China. Electronic address: yang_rya@sina.com.
Abstract
BACKGROUND/ PURPOSE: Silver nanoparticles are receiving increasing attention in biomedical applications. This study aims at evaluating the antifungal properties of silver nanoparticles against the pathogenic fungus Trichosporon asahii. METHODS: The growth of T. asahii on potato dextrose agar medium containing different concentrations of silver nanoparticles was examined and the antifungal effect was evaluated using minimum inhibitory concentration. Scanning and transmission electron microscopy were also used to investigate the antifungal effect of silver nanoparticles on T. asahii. RESULTS: Silver nanoparticles had a significant inhibitory effect on the growth of T. asahii. The minimum inhibitory concentration of silver nanoparticles against T. asahii was 0.5 μg/mL, which was lower than amphotericin B, 5-flucytosine, caspofungin, terbinafine, fluconazole, and itraconazole and higher than voriconazole. Silver nanoparticles obviously damaged the cell wall, cell membrane, mitochondria, chromatin, and ribosome. CONCLUSION: Our results demonstrate that silver nanoparticles have good antifungal activity against T. asahii. Based on our electron microscopy observations, silver nanoparticles may inhibit the growth of T. asahii by permeating the fungal cell and damaging the cell wall and cellular components.
BACKGROUND/ PURPOSE:Silver nanoparticles are receiving increasing attention in biomedical applications. This study aims at evaluating the antifungal properties of silver nanoparticles against the pathogenic fungus Trichosporon asahii. METHODS: The growth of T. asahii on potato dextrose agar medium containing different concentrations of silver nanoparticles was examined and the antifungal effect was evaluated using minimum inhibitory concentration. Scanning and transmission electron microscopy were also used to investigate the antifungal effect of silver nanoparticles on T. asahii. RESULTS:Silver nanoparticles had a significant inhibitory effect on the growth of T. asahii. The minimum inhibitory concentration of silver nanoparticles against T. asahii was 0.5 μg/mL, which was lower than amphotericin B, 5-flucytosine, caspofungin, terbinafine, fluconazole, and itraconazole and higher than voriconazole. Silver nanoparticles obviously damaged the cell wall, cell membrane, mitochondria, chromatin, and ribosome. CONCLUSION: Our results demonstrate that silver nanoparticles have good antifungal activity against T. asahii. Based on our electron microscopy observations, silver nanoparticles may inhibit the growth of T. asahii by permeating the fungal cell and damaging the cell wall and cellular components.
Authors: Mousa A Alghuthaymi; Kamel A Abd-Elsalam; Ashwag Shami; Ernest Said-Galive; Eleonora V Shtykova; Alexander V Naumkin Journal: J Fungi (Basel) Date: 2020-04-19
Authors: Francis J Osonga; Ali Akgul; Idris Yazgan; Ayfer Akgul; Gaddi B Eshun; Laura Sakhaee; Omowunmi A Sadik Journal: Molecules Date: 2020-06-09 Impact factor: 4.411