Yan Jia1, Dan-Ning Hu2, Dongqing Zhu1, Leilei Zhang1, Ping Gu1, Xianqun Fan1, Jibo Zhou1. 1. Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, People's Republic of China. 2. Departments of Ophthalmology and Pathology, New York Eye and Ear Infirmary, New York, New York, United States.
Abstract
PURPOSE: We measured the aqueous humor levels of matrix metalloproteinases (MMP) MMP-1, MMP-2, MMP-3, and tissue inhibitors of matrix metalloproteinases (TIMP) TIMP-1, TIMP-2, and TIMP-3 in patients with myopia or cataract, and investigated the relationship between their levels and axial length (AL). METHODS: We measured MMP/TIMPs levels with the Luminex xMAP Technology by using commercially available Milliplex xMAP Kits. A total of 65 aqueous humor samples was collected from patients with myopia or cataract during cataract or clear lens extraction surgery. According to the AL, the samples were divided into three groups: group A, AL ≤ 24 mm; group B, AL 24 to 26 mm; and group C, AL ≥ 26 mm. RESULTS: Levels of MMP-2, MMP-3, TIMP-1, TIMP-2, and TIMP-3 could be detected in the aqueous humor. The levels of MMP-2, TIMP-1, TIMP-2, and TIMP-3 were positively correlated with AL. The differences of the levels of these MMPs/TIMPs among the three groups were statistically significant. The MMP-3 levels were not correlated with AL and there was no significant difference in MMP-3 levels among these three groups. Levels of MMP-1 could not be detected in the aqueous humor samples. CONCLUSIONS: Elevated levels of aqueous MMP-2, TIMP-1, TIMP-2, and TIMP-3 were found in the eyes with elongated axis. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
PURPOSE: We measured the aqueous humor levels of matrix metalloproteinases (MMP) MMP-1, MMP-2, MMP-3, and tissue inhibitors of matrix metalloproteinases (TIMP) TIMP-1, TIMP-2, and TIMP-3 in patients with myopia or cataract, and investigated the relationship between their levels and axial length (AL). METHODS: We measured MMP/TIMPs levels with the Luminex xMAP Technology by using commercially available Milliplex xMAP Kits. A total of 65 aqueous humor samples was collected from patients with myopia or cataract during cataract or clear lens extraction surgery. According to the AL, the samples were divided into three groups: group A, AL ≤ 24 mm; group B, AL 24 to 26 mm; and group C, AL ≥ 26 mm. RESULTS: Levels of MMP-2, MMP-3, TIMP-1, TIMP-2, and TIMP-3 could be detected in the aqueous humor. The levels of MMP-2, TIMP-1, TIMP-2, and TIMP-3 were positively correlated with AL. The differences of the levels of these MMPs/TIMPs among the three groups were statistically significant. The MMP-3 levels were not correlated with AL and there was no significant difference in MMP-3 levels among these three groups. Levels of MMP-1 could not be detected in the aqueous humor samples. CONCLUSIONS: Elevated levels of aqueous MMP-2, TIMP-1, TIMP-2, and TIMP-3 were found in the eyes with elongated axis. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
Authors: Gayle B Collin; Dirk Hubmacher; Jeremy R Charette; Wanda L Hicks; Lisa Stone; Minzhong Yu; Jürgen K Naggert; Mark P Krebs; Neal S Peachey; Suneel S Apte; Patsy M Nishina Journal: Hum Mol Genet Date: 2015-09-24 Impact factor: 6.150