Min Wang1, Xudong Xu1, Huibo Xu2, Fuchun Wen2, Xiaopo Zhang1, Hong Sun1, Fan Yao3, Guibo Sun4, Xiaobo Sun5. 1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. 2. Academy of Chinese Medical Sciences of Jilin Province, Gongnongda road 1745, Changchun 130021, Jilin, PR China. 3. School of Biological Sciences, University of Edinburgh, King׳s Buildings, Edinburgh EH9 3JU, UK. 4. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. Electronic address: sunguibo@126.com. 5. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, PR China. Electronic address: sunsubmit@163.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Total saponins of Aralia elata (Miq) Seem (AS) from the Chinese traditional herb Longya Aralia chinensis L. can improve cardiac function, although the active mechanism remains poorly understood. The present study aimed to determine the direct effect of AS on cardiac function in dogs and the effects on Ca2+ transient and contractions in isolated rat cardiomyocytes. MATERIAL AND METHODS: In anesthetized dogs, hemodynamic indexes and myocardial oxygen consumption were determined before and after AS was administered. In isolated adult rat cardiomyocytes, contractile and intracellular Ca2+ properties were determined simultaneously in real time by using an IonOptix MyoCam system. RESULTS: Our results showed that AS directly induced a positive inotropic effect and improved coronary blood flow and energy metabolism, indicating that AS induced a beneficial effect to treat myocardial ischemia/reperfusion injury. Moreover, AS increased sarcomere shortening, maximal velocity of shortening/relengthening (±dL/dt), amplitude of [Ca2+]i transients and SERCA activity in a concentration-dependent manner. PKCε was also activated after the cells were treated with AS. CONCLUSION: These findings revealed the positive inotropic effect of AS on canine myocardium and isolated rat cardiomyocytes. This effect was possibly associated with an increase in amplitude of the [Ca2+]i transient and PKCε-dependent signaling pathway.
ETHNOPHARMACOLOGICAL RELEVANCE: Total saponins of Aralia elata (Miq) Seem (AS) from the Chinese traditional herb Longya Aralia chinensis L. can improve cardiac function, although the active mechanism remains poorly understood. The present study aimed to determine the direct effect of AS on cardiac function in dogs and the effects on Ca2+ transient and contractions in isolated rat cardiomyocytes. MATERIAL AND METHODS: In anesthetized dogs, hemodynamic indexes and myocardial oxygen consumption were determined before and after AS was administered. In isolated adult rat cardiomyocytes, contractile and intracellular Ca2+ properties were determined simultaneously in real time by using an IonOptix MyoCam system. RESULTS: Our results showed that AS directly induced a positive inotropic effect and improved coronary blood flow and energy metabolism, indicating that AS induced a beneficial effect to treat myocardial ischemia/reperfusion injury. Moreover, AS increased sarcomere shortening, maximal velocity of shortening/relengthening (±dL/dt), amplitude of [Ca2+]i transients and SERCA activity in a concentration-dependent manner. PKCε was also activated after the cells were treated with AS. CONCLUSION: These findings revealed the positive inotropic effect of AS on canine myocardium and isolated rat cardiomyocytes. This effect was possibly associated with an increase in amplitude of the [Ca2+]i transient and PKCε-dependent signaling pathway.
Authors: Jing-Yi Zhang; Gui-Bo Sun; Min Wang; Ping Liao; Yu-Yang Du; Ke Yang; Xiao-Bo Sun Journal: Toxicol Res (Camb) Date: 2016-02-08 Impact factor: 3.524