| Literature DB >> 24873745 |
Feng Yang1, Yaoju Tan2, Jia Liu1, Tianzhou Liu3, Bangxing Wang4, Yuanyuan Cao4, Yue Qu5, Trevor Lithgow5, Shouyong Tan2, Tianyu Zhang6.
Abstract
The genetic study of mycobacteria, such as Mycobacterium tuberculosis and Mycobacterium ulcerans, is hampered heavily by their slow growth. We have developed efficient, versatile, and improved genetic tools for constructing unmarked recombinant mycobacteria more rapidly including generating multiple mutants using the same antibiotic marker in both fast- and slow-growing mycobacteria.Entities:
Keywords: In-frame deletion; Mycobacteria; Recombineering; Unmarked gene disruption; Xer recombinase system
Mesh：
Year: 2014 PMID： 24873745 DOI： 10.1016/j.mimet.2014.05.007
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363