Hermán Pinto1, David Ricart-Jané2, Eva Pardina2. 1. Instituto de Investigaciones para las Especialidades Esteticas y del Envejecimiento, Barcelona. info@i2e3.com). 2. Centre de Recerca en Metabolisme, Barcelona (CEREMET).
Abstract
BACKGROUND: New knowledge about crystallization vs. lipid-to-gel transition has surfaced recently, since some of the latest publications on lipocryolysis have focused on its action mechanism. As a result, new opportunities for technical improvements and clinical outcome optimization have opened up. The food industry has been working with lipid crystal polymorphisms for decades, and tempering seems to be the easiest method of external conditioning, in addition to being harmless. OBJECTIVE: Evaluate if pre and post lipocryolyisis thermic conditioning enhances rat adipocyte destruction. METHODS: Several temperature treatment patterns (TTP) were applied to isolated rat adipocytes. The survival of the adipocytes exposed to the different TTPs and the formation of crystals in the surviving adipocytes were assessed and analyzed. RESULT: Pre and post lipocryolysis thermic conditioning changed lipocryolyisis crystallization process and showed an enhancement in adipocyte destruction that could represent an important step in improving clinical results. CONCLUSIONS: pre and post lipocryolyisis thermic conditioning enhances rat adipocyte destruction.
BACKGROUND: New knowledge about crystallization vs. lipid-to-gel transition has surfaced recently, since some of the latest publications on lipocryolysis have focused on its action mechanism. As a result, new opportunities for technical improvements and clinical outcome optimization have opened up. The food industry has been working with lipid crystal polymorphisms for decades, and tempering seems to be the easiest method of external conditioning, in addition to being harmless. OBJECTIVE: Evaluate if pre and post lipocryolyisis thermic conditioning enhances rat adipocyte destruction. METHODS: Several temperature treatment patterns (TTP) were applied to isolated rat adipocytes. The survival of the adipocytes exposed to the different TTPs and the formation of crystals in the surviving adipocytes were assessed and analyzed. RESULT: Pre and post lipocryolysis thermic conditioning changed lipocryolyisis crystallization process and showed an enhancement in adipocyte destruction that could represent an important step in improving clinical results. CONCLUSIONS: pre and post lipocryolyisis thermic conditioning enhances rat adipocyte destruction.