Ahmet Kocael1, Hayriye Erman2, Kagan Zengin3, Pinar C A Kocael1, Gulcan G Korkmaz4, Remise Gelisgen2, Mustafa Taskin1, Yilmaz Ersan1, Hafize Uzun2. 1. The Department of General Surgery, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 2. The Department of Biochemistry, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 3. The Department of General Surgery, UCerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 4. Kirklareli University, School of Health, Kirklareli, Turkey.
Abstract
BACKGROUND: Obesity may induce oxidative stress, causing oxidative damage of DNA. We examined associations between decreasing serum and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels and weight loss in morbidly obese patients before and 6 months after laparoscopic adjustable gastric banding (LAGB). METHODS: We compared patients who had surgery for morbid obesity with healthy, nonobese controls. Urine and fasting blood samples were collected once from the controls and from the morbidly obese patients before and 6 months after the LAGB. The serum and urinary 8-OHdG levels were evaluated in these groups using an enzyme-linked immunosorbent assay kit. RESULTS: We included 20 patients who had surgery for morbid obesity (8 men, 12 women, mean body mass index [BMI] 46.82 ± 4.47) and 20 healthy, nonobese people (10 men, 10 women, mean BMI 22.52 ± 2.08) in our study. There was no significant difference in serum 8-OHdG levels between the groups, whereas urinary 8- OHdG levels were significantly higher in morbidly obese patients than in controls. Weight, BMI and serum and urinary 8-OHdG levels were significantly decreased in morbidly obese patients 6 months after LAGB. CONCLUSION: The LAGB provides efficient weight loss in patients with morbid obesity. The systemic oxidative DNA damage was increased by the morbid obesity, but this increase was not related to weight gain, and it was more evident in serum than urine samples. After LAGB for morbid obesity, the oxidative DNA damage declined both in serum and urine.
BACKGROUND:Obesity may induce oxidative stress, causing oxidative damage of DNA. We examined associations between decreasing serum and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels and weight loss in morbidly obesepatients before and 6 months after laparoscopic adjustable gastric banding (LAGB). METHODS: We compared patients who had surgery for morbid obesity with healthy, nonobese controls. Urine and fasting blood samples were collected once from the controls and from the morbidly obesepatients before and 6 months after the LAGB. The serum and urinary 8-OHdG levels were evaluated in these groups using an enzyme-linked immunosorbent assay kit. RESULTS: We included 20 patients who had surgery for morbid obesity (8 men, 12 women, mean body mass index [BMI] 46.82 ± 4.47) and 20 healthy, nonobese people (10 men, 10 women, mean BMI 22.52 ± 2.08) in our study. There was no significant difference in serum 8-OHdG levels between the groups, whereas urinary 8- OHdG levels were significantly higher in morbidly obesepatients than in controls. Weight, BMI and serum and urinary 8-OHdG levels were significantly decreased in morbidly obesepatients 6 months after LAGB. CONCLUSION: The LAGB provides efficient weight loss in patients with morbid obesity. The systemic oxidative DNA damage was increased by the morbid obesity, but this increase was not related to weight gain, and it was more evident in serum than urine samples. After LAGB for morbid obesity, the oxidative DNA damage declined both in serum and urine.
Authors: Andrzej R Trzeciak; Simon G Nyaga; Pawel Jaruga; Althaf Lohani; Miral Dizdaroglu; Michele K Evans Journal: Carcinogenesis Date: 2004-03-25 Impact factor: 4.944
Authors: Lidia Monzo-Beltran; Antonio Vazquez-Tarragón; Concha Cerdà; Paula Garcia-Perez; Antonio Iradi; Carlos Sánchez; Benjamin Climent; Carmen Tormos; Antonio Vázquez-Prado; Javier Girbés; Nuria Estáñ; Sebastián Blesa; Raquel Cortés; Felipe J Chaves; Guillermo T Sáez Journal: Redox Biol Date: 2017-02-14 Impact factor: 11.799