BACKGROUND: Neural crest cells are multipotent cells that migrate extensively throughout vertebrate embryos to form diverse lineages. Cell migration requires polarized, organized actin networks that provide the driving force for motility. Actin-binding proteins that regulate neural crest cell migration are just beginning to be defined. RESULTS: We recently identified a number of actin-associated factors through proteomic profiling of methylated proteins in migratory neural crest cells. Here, we report the previously undocumented expression pattern of three of these proteins in chick early neural crest development: doublecortin (DCX), tropomyosin-1 (TPM-1), and actin depolymerizing factor (ADF). All three genes are expressed with varying degrees of specificity and intensity in premigratory and migratory neural crest cells, and their resulting proteins exhibit distinct subcellular localization in migratory neural crest cells. Morpholino knock down of ADF reveals it is required for Sox10 gene expression, but minimally important during neural crest migration. CONCLUSIONS: Neural crest cells express DCX, TPM-1, and ADF. ADF is necessary during neural crest specification, but largely dispensable for migration.
BACKGROUND: Neural crest cells are multipotent cells that migrate extensively throughout vertebrate embryos to form diverse lineages. Cell migration requires polarized, organized actin networks that provide the driving force for motility. Actin-binding proteins that regulate neural crest cell migration are just beginning to be defined. RESULTS: We recently identified a number of actin-associated factors through proteomic profiling of methylated proteins in migratory neural crest cells. Here, we report the previously undocumented expression pattern of three of these proteins in chick early neural crest development: doublecortin (DCX), tropomyosin-1 (TPM-1), and actin depolymerizing factor (ADF). All three genes are expressed with varying degrees of specificity and intensity in premigratory and migratory neural crest cells, and their resulting proteins exhibit distinct subcellular localization in migratory neural crest cells. Morpholino knock down of ADF reveals it is required for Sox10 gene expression, but minimally important during neural crest migration. CONCLUSIONS: Neural crest cells express DCX, TPM-1, and ADF. ADF is necessary during neural crest specification, but largely dispensable for migration.
Authors: Rebecca McLennan; Louise Dyson; Katherine W Prather; Jason A Morrison; Ruth E Baker; Philip K Maini; Paul M Kulesa Journal: Development Date: 2012-07-04 Impact factor: 6.868
Authors: Wilma A Hofmann; Ljuba Stojiljkovic; Beata Fuchsova; Gabriela M Vargas; Evangelos Mavrommatis; Vlada Philimonenko; Katarina Kysela; James A Goodrich; James L Lessard; Thomas J Hope; Pavel Hozak; Primal de Lanerolle Journal: Nat Cell Biol Date: 2004-10-24 Impact factor: 28.824
Authors: Darwin Martinez; Nora Zuhdi; Michelle Reyes; Blanca Ortega; Dion Giovannone; Vivian M Lee; Maria Elena de Bellard Journal: Gene Expr Patterns Date: 2018-02-07 Impact factor: 1.224
Authors: Julia Welzenbach; Nigel L Hammond; Miloš Nikolić; Frederic Thieme; Nina Ishorst; Elizabeth J Leslie; Seth M Weinberg; Terri H Beaty; Mary L Marazita; Elisabeth Mangold; Michael Knapp; Justin Cotney; Alvaro Rada-Iglesias; Michael J Dixon; Kerstin U Ludwig Journal: HGG Adv Date: 2021-06-08