Eliana R Steinberg1, Mariela Nieves, Lucía Fantini, Marta D Mudry. 1. Grupo de Investigación en Biología Evolutiva (GIBE), Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IEGEBA (CONICET-UBA), Ciudad Universitaria, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Abstract
BACKGROUND: Captive primates are often maintained in groups without geographic origin or genetic heritage information. This could lead to an incorrect assignment of species, which could result in an inadequate management of the colonies. METHODS: We present a cytogenetic protocol adapted to be successfully used in an accurate taxonomic diagnosis of non-human primates (Platyrrhini), including lymphocyte culture, G- and C-banding, meiosis, and fluorescent in situ hybridization technique (FISH). RESULTS: Using classical cytogenetic diagnosis, the species status was determined in 541 Platyrrhini individuals. Of these, 99 were previously erroneously sexed or assigned to a different species using only morphological characteristics. CONCLUSIONS: The cytogenetic results highlight the relevance of the genetic characterization of primates both in captivity and in the wild. These techniques had been used in our research group for more than 30 years in different research projects, not only for characterizing hundreds of primates, but also different for topics regarding primates genomes and evolution.
BACKGROUND: Captive primates are often maintained in groups without geographic origin or genetic heritage information. This could lead to an incorrect assignment of species, which could result in an inadequate management of the colonies. METHODS: We present a cytogenetic protocol adapted to be successfully used in an accurate taxonomic diagnosis of non-human primates (Platyrrhini), including lymphocyte culture, G- and C-banding, meiosis, and fluorescent in situ hybridization technique (FISH). RESULTS: Using classical cytogenetic diagnosis, the species status was determined in 541 Platyrrhini individuals. Of these, 99 were previously erroneously sexed or assigned to a different species using only morphological characteristics. CONCLUSIONS: The cytogenetic results highlight the relevance of the genetic characterization of primates both in captivity and in the wild. These techniques had been used in our research group for more than 30 years in different research projects, not only for characterizing hundreds of primates, but also different for topics regarding primates genomes and evolution.
Authors: Diego Mattos Penedo; Jorge Luís Azevedo de Armada; Mariela Nieves; Carlos Eduardo da Silva Verona; Andréa Maria de Oliveira; Emidio José de Sousa Dos Santos; Denise Monnerat Nogueira Journal: Primates Date: 2021-01-18 Impact factor: 2.163