Jonatas Rafael de Oliveira1, Rosilene Batista de Aguiar Almeida2, Polyana das Graças Figueiredo Vilela2, Felipe Eduardo de Oliveira2, Rosilene Fernandes da Rocha3, Antonio Olavo Cardoso Jorge2, Luciane Dias de Oliveira3. 1. Laboratory of Microbiology and Immunology, Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Universidade Estadual Paulista/UNESP, São José dos Campos, SP, Brazil. Electronic address: jroliveira16@hotmail.com. 2. Laboratory of Microbiology and Immunology, Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Universidade Estadual Paulista/UNESP, São José dos Campos, SP, Brazil. 3. Laboratory of Biochemistry and Pharmacology, Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Universidade Estadual Paulista/UNESP, São José dos Campos, SP, Brazil.
Abstract
OBJECTIVES: To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7). DESIGN: By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1β and TNF-α by ELISA. RESULTS: The most effective concentration was 250 mg/mL and also promoted significant reduction (log₁₀) in the biofilms of S. aureus (0.438 ± 0.269), S. epidermidis (0.377 ± 0.298), S. mutans (0.244 ± 0.161) and C. albicans (0.746 ± 0.209). Cell viability was similar to 100%. The production of IL-1β was similar to the control group (p>0.05) and there was inhibition of TNF-α (p<0.01). CONCLUSIONS: A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages.
OBJECTIVES: To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7). DESIGN: By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicityA. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1β and TNF-α by ELISA. RESULTS: The most effective concentration was 250 mg/mL and also promoted significant reduction (log₁₀) in the biofilms of S. aureus (0.438 ± 0.269), S. epidermidis (0.377 ± 0.298), S. mutans (0.244 ± 0.161) and C. albicans (0.746 ± 0.209). Cell viability was similar to 100%. The production of IL-1β was similar to the control group (p>0.05) and there was inhibition of TNF-α (p<0.01). CONCLUSIONS:A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages.
Authors: Lucas Paula-Ramos; Carlos Eduardo da Rocha Santos; Daphne Camargo Reis Mello; Lígia Nishiama Theodoro; Felipe Eduardo De Oliveira; Graziella N Back Brito; Juliana Campos Junqueira; Antonio Olavo Cardoso Jorge; Luciane Dias de Oliveira Journal: ScientificWorldJournal Date: 2016-11-28
Authors: D Jesus; J R Oliveira; F E Oliveira; K C Higa; J C Junqueira; A O C Jorge; G N Back-Brito; L D Oliveira Journal: ScientificWorldJournal Date: 2015-10-29
Authors: João Paulo Mendes Tribst; Amanda Maria de Oliveira Dal Piva; Dimas Renno de Lima; Alexandre Luiz Souto Borges; Marco Antonio Bottino Journal: J Indian Soc Periodontol Date: 2018 Jul-Aug