Stefano de Franciscis1, Raffaele Grande2, Lucia Butrico2, Gianluca Buffone3, Luca Gallelli4, Edoardo Scarcello5, Francesco Giuseppe Caliò6, Domenico De Vito7, Rita Compagna8, Maurizio Amato7, Francesco Fugetto9, Vincenzo Gasbarro10, Bruno Amato8, Raffaele Serra11. 1. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, Headquarters: University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy; Department of Medical and Surgical Sciences, University of Catanzaro, Italy. 2. Department of Medical and Surgical Sciences, University of Catanzaro, Italy. 3. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, Headquarters: University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy. 4. Department of Health Science, University of Catanzaro, Italy. 5. Unit of Vascular Surgery, Annunziata Hospital, Cosenza, Italy. 6. Unit of Vascular Surgery, S. Anna Hospital, Catanzaro, Italy. 7. Department of Clinical Medicine and Surgery, University of Naples "Federico II", Italy. 8. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, Headquarters: University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy; Department of Clinical Medicine and Surgery, University of Naples "Federico II", Italy. 9. University of Modena and Reggio Emilia, Corso Canal Grande, 41011 Modena, Italy. 10. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, Headquarters: University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy; Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Italy. 11. Interuniversity Center of Phlebolymphology (CIFL), International Research and Educational Program in Clinical and Experimental Biotechnology, Headquarters: University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy; Department of Medical and Surgical Sciences, University of Catanzaro, Italy. Electronic address: rserra@unicz.it.
Abstract
INTRODUCTION: Carotid Body Tumors (CBTs) are Paragangliomas (PGLs) located in the head and neck region which usually do not cause overt neuroendocrine symptoms and hypertension. Matrix Metalloproteinases (MMPs) have shown a strong correlation between CBTs and their clinical behavior. Aim of this study is to analyze the relationship between changes in arterial blood pressure and metalloproteinases levels after surgical resection of CBTs. METHODS: We performed a multicenter clinical study on 17 patients with benign and malignant CBTs (5 males; 12 females). Tumors were completely resected and biopsies, obtained at the time of surgery, were lysed for Western blot analysis to determine MMPs levels in tissues. An enzyme-linked immune sorbent assay (ELISA) kit was used to determine the concentration of MMPs in plasma fluid. Blood pressure values were measured at admission and at 10 days after surgery. RESULTS: At the time of the admission, blood pressure values were higher in patients with CBTs respect to control patients; moreover in patients with malignant CBTs blood pressure values were higher (P < 0.01) respect to patients with benign CBTs. 10 days after the surgery, we documented a significant decrease (P < 0.01) in blood pressure values and in MMPs levels in all patients with CBTs. CONCLUSION: These results suggest that, despite the CTBs are considered non-functional tumors, an "underestimated" neuroendocrine activity on arterial blood pressure may be detected.
INTRODUCTION:Carotid Body Tumors (CBTs) are Paragangliomas (PGLs) located in the head and neck region which usually do not cause overt neuroendocrine symptoms and hypertension. Matrix Metalloproteinases (MMPs) have shown a strong correlation between CBTs and their clinical behavior. Aim of this study is to analyze the relationship between changes in arterial blood pressure and metalloproteinases levels after surgical resection of CBTs. METHODS: We performed a multicenter clinical study on 17 patients with benign and malignant CBTs (5 males; 12 females). Tumors were completely resected and biopsies, obtained at the time of surgery, were lysed for Western blot analysis to determine MMPs levels in tissues. An enzyme-linked immune sorbent assay (ELISA) kit was used to determine the concentration of MMPs in plasma fluid. Blood pressure values were measured at admission and at 10 days after surgery. RESULTS: At the time of the admission, blood pressure values were higher in patients with CBTs respect to control patients; moreover in patients with malignant CBTs blood pressure values were higher (P < 0.01) respect to patients with benign CBTs. 10 days after the surgery, we documented a significant decrease (P < 0.01) in blood pressure values and in MMPs levels in all patients with CBTs. CONCLUSION: These results suggest that, despite the CTBs are considered non-functional tumors, an "underestimated" neuroendocrine activity on arterial blood pressure may be detected.
Authors: Marat Fudim; Kelly L Groom; Cheryl L Laffer; James L Netterville; David Robertson; Fernando Elijovich Journal: J Am Soc Hypertens Date: 2015-03-19
Authors: Giovanni De Caridi; Mafalda Massara; Francesco Stilo; Francesco Spinelli; Raffaele Grande; Lucia Butrico; Stefano de Franciscis; Raffaele Serra Journal: Int Wound J Date: 2014-08-05 Impact factor: 3.315
Authors: Diego José Rojas Esquivel; Stefan Stefanov Kiuri; Alvaro Fernández Heredero; Luis Riera De Cubas; José Cebrian Carretero Journal: SAGE Open Med Case Rep Date: 2014-11-19
Authors: Giovanni De Caridi; Mafalda Massara; Francesco Spinelli; Raffaele Grande; Lucia Butrico; Pierandrea Rende; Maurizio Amato; Rita Compagna; Bruno Amato; Stefano de Franciscis; Raffaele Serra Journal: Open Med (Wars) Date: 2015-12-17
Authors: Aldo Rocca; Domenico Tafuri; Marianna Paccone; Antonio Giuliani; Anna Ginevra Immacolata Zamboli; Giuseppe Surfaro; Andrea Paccone; Rita Compagna; Maurizo Amato; Raffaele Serra; Bruno Amato Journal: Open Med (Wars) Date: 2017-10-21