RATIONALE: Visualization of the spatial distribution of phosphatidylcholine (PC) in tissues by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) provides insights into key physiological and pathophysiological processes. In MALDI-IMS analysis, the heterogeneity of adduct ions formed from PC lowers the specificity of detection of PC molecular species and poses a challenge in the identification of these species. To solve this problem, modified matrix solution and desalting with ammonium acetate (NH4 Ac) buffer have been employed. However, the utility of these methods is limited to the analysis of brain sections. METHODS: The MALDI signal intensities of [PC+H], [PC+Na] and [PC+K] were compared after three different pretreatments (modified matrix solution, desalting with 150 mM ammonium acetate, treatment with 150 mM potassium acetate). RESULTS: Pretreatment of tissue sections with 150 mM potassium acetate resulted in an increase in the signal intensity of [PC+K] ions produced from cryosections of the pancreas, brain, and liver tissues. CONCLUSIONS: Pretreatment with potassium acetate can be a simple, improved, and highly useful method for the reliable analysis of PC in tissues.
RATIONALE: Visualization of the spatial distribution of phosphatidylcholine (PC) in tissues by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) provides insights into key physiological and pathophysiological processes. In MALDI-IMS analysis, the heterogeneity of adduct ions formed from PC lowers the specificity of detection of PC molecular species and poses a challenge in the identification of these species. To solve this problem, modified matrix solution and desalting with ammonium acetate (NH4Ac) buffer have been employed. However, the utility of these methods is limited to the analysis of brain sections. METHODS: The MALDI signal intensities of [PC+H], [PC+Na] and [PC+K] were compared after three different pretreatments (modified matrix solution, desalting with 150 mM ammonium acetate, treatment with 150 mM potassium acetate). RESULTS: Pretreatment of tissue sections with 150 mM potassium acetate resulted in an increase in the signal intensity of [PC+K] ions produced from cryosections of the pancreas, brain, and liver tissues. CONCLUSIONS: Pretreatment with potassium acetate can be a simple, improved, and highly useful method for the reliable analysis of PC in tissues.
Authors: Jacob X M Truong; Xander Spotbeen; Jake White; Johannes V Swinnen; Lisa M Butler; Marten F Snel; Paul J Trim Journal: Anal Bioanal Chem Date: 2021-02-09 Impact factor: 4.142
Authors: Amy V Walker; Lev D Gelb; Grant E Barry; Polatip Subanajouy; Ananta Poudel; Manami Hara; Igor V Veryovkin; Graeme I Bell; Luke Hanley Journal: Biointerphases Date: 2018-04-02 Impact factor: 2.456