Marize Quinhones Pires1, Maria de Fátima Madeira2, Vânia Rita Elias Pinheiro Bittencourt3, Raquel da Silva Pacheco1. 1. Laboratório de Sistemática Bioquímica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. 2. Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. 3. Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ.
Abstract
INTRODUCTION: During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. METHODS: Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. RESULTS: PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. CONCLUSIONS: This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.
INTRODUCTION: During a diagnostic evaluation of caninevisceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. METHODS: Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. RESULTS: PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. CONCLUSIONS: This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.
Authors: Luciana de Freitas Campos Miranda; Raquel da Silva Pacheco; Maria Inês Fernandes Pimentel; Mariza de Matos Salgueiro; Aline Fagundes da Silva; Cíntia Xavier de Mello; Juliana Helena da Silva Barros; Claudia Maria Valete-Rosalino; Maria de Fátima Madeira; Samanta Cristina das Chagas Xavier; Armando de Oliveira Schubach Journal: PLoS Negl Trop Dis Date: 2019-11-15
Authors: Luz H Patiño; Adriana C Castillo-Castañeda; Marina Muñoz; Jesus E Jaimes; Nicolas Luna-Niño; Carolina Hernández; Martha S Ayala; Patricia Fuya; Claudia Mendez; Carlos E Hernández-Pereira; Lourdes Delgado; Claudia M Sandoval-Ramírez; Plutarco Urbano; Alberto Paniz-Mondolfi; Juan David Ramírez Journal: Microbiol Spectr Date: 2021-10-13