Literature DB >> 24858450

Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR.

Sizhong Yang1, Susanne Liebner2, Mashal Alawi2, Oliver Ebenhöh2, Dirk Wagner2.   

Abstract

The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the MOTHUR software.
Copyright © 2014 Elsevier B.V. All rights reserved.

Keywords:  Cut-off; MOTHUR; Methanogens; Taxonomic database; mcrA gene

Mesh：

Substances：

Year:  2014        PMID： 24858450     DOI： 10.1016/j.mimet.2014.05.006

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


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