| Literature DB >> 24856455 |
Wenyan Shi1, Qing Liu1, Jie Zhang1, Jingjing Sun1, Xiyue Jiang1, Jing Geng1, Fangkun Wang1, Yihong Xiao2, Hongmei Li3, Xiaomin Zhao4.
Abstract
In the present study, a naked EtMIC2 DNA vaccine, a ChIL-18 expression vector and a EtMIC2 and ChIL-18 co-expression DNA vaccine were constructed and their protective efficacies against homologous challenge were compared and evaluated by examining the body weight gain, oocyst shedding, cecal lesion, ACI as well as specific anti-EtMic2 antibody level, the proliferation ability and percentages of CD4+ and CD8+ of splenocytes. The results showed the naked EtMIC2 DNA vaccine could increase the weight gain and decrease the oocyst shedding, but could not alleviate the cecal lesion of immunized chickens compared to unimmunized chickens. Chickens immunized with the co-expression vector pVAX1-MIC2-IL-18 exhibited much improved immune protection against challenge compared to chickens immunized with naked EtMIC2 DNA vaccine, or with naked EtMIC2 DNA vaccine and ChIL-18 expression vector applied separately. These results suggest that the co-expression of ChIL-18 with EtMic2 together could significantly improve the immune protection of the EtMic2 protein.Entities:
Keywords: ChIL-18; Co-expression; DNA vaccine; EtMIC2
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Year: 2014 PMID： 24856455 DOI： 10.1016/j.rvsc.2014.05.001
Source DB: PubMed Journal: Res Vet Sci ISSN： 0034-5288 Impact factor: 2.534