Hui Li1, Caiqing Zhang2, Ping Liu1, Weiguo Liu3, Yuan Gao1, Shujuan Sun4. 1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong Province, China. 2. Department of Respiratory Medicine, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China. 3. Department of Pharmacy, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China. 4. Department of Pharmacy, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong Province, China. Electronic address: sunshujuan888@163.com.
Abstract
BACKGROUND/ PURPOSE: It is difficult to manage coinfections in critically ill patients, especially in the presence of mixed-species biofilms. The aim of this study was to seek an effective drug combination for managing the dual-species biofilm of Candida albicans and Staphylococcus aureus. METHODS: The interaction between fluconazole and minocycline against polymicrobial planktonic cells and polymicrobial biofilms formed over four different time intervals (4 hours, 8 hours, 12 hours, and 24 hours) was investigated using a microdilution checkerboard method. To explore whether the combined effects against the polymicrobial cultures involved calcium regulation, the effects of benidipine and ethylene glycol tetraacetic acid were characterized using a plate streaking method and a liquid-based quantitative method. RESULTS: Fluconazole combined with minocycline exerted strong effects against polymicrobial planktonic cells and polymicrobial biofilms formed over 4 hours, 8 hours, and 12 hours. The addition of benidipine and ethylene glycol tetraacetic acid enhanced the activity of the drug combination, suggesting that the combined effects may involve the perturbation of calcium homeostasis. CONCLUSION: Fluconazole in combination with minocycline is a potential approach for counteracting C. albicans-S. aureus dual-species biofilms.
BACKGROUND/ PURPOSE: It is difficult to manage coinfections in critically illpatients, especially in the presence of mixed-species biofilms. The aim of this study was to seek an effective drug combination for managing the dual-species biofilm of Candida albicans and Staphylococcus aureus. METHODS: The interaction between fluconazole and minocycline against polymicrobial planktonic cells and polymicrobial biofilms formed over four different time intervals (4 hours, 8 hours, 12 hours, and 24 hours) was investigated using a microdilution checkerboard method. To explore whether the combined effects against the polymicrobial cultures involved calcium regulation, the effects of benidipine and ethylene glycol tetraacetic acid were characterized using a plate streaking method and a liquid-based quantitative method. RESULTS:Fluconazole combined with minocycline exerted strong effects against polymicrobial planktonic cells and polymicrobial biofilms formed over 4 hours, 8 hours, and 12 hours. The addition of benidipine and ethylene glycol tetraacetic acid enhanced the activity of the drug combination, suggesting that the combined effects may involve the perturbation of calcium homeostasis. CONCLUSION:Fluconazole in combination with minocycline is a potential approach for counteracting C. albicans-S. aureus dual-species biofilms.
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