Jie Chen1, Maitea Guridi2, Melinda E Fernyhough3, Zhihua Jiang4, LeLuo Guan5, Gary J Hausman6, Michael V Dodson4. 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China ; Department of Animal Science, Washington State University, Pullman, WA 99164. 2. Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona 31006 Navarra, Spain. 3. The Hartz Mountain Corporation, Bloomfield, NJ 07003. 4. Department of Animal Science, Washington State University, Pullman, WA 99164. 5. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5. 6. USDA-ARS, Richard B. Russell Agricultural Research Station, Athens, GA, 30604.
Abstract
BACKGROUND AND OBJECTIVES: Numerous in vitro observations have been published to show that mature adipocytes may resume proliferation and begin to populate the adipofibroblast fraction or form other cell types. METHODS AND RESULTS: In the present study, we evaluated clonal cultures of mature pig-derived adipocytes as they began to reestablish their ability to divide. The lipid contained within the cytoplasm was either moved to the apical ends of the cell, or large droplets were physically extruded from the cell. In the latter case, we ascertained that the cell lipid droplet was handled in a different manner to that by beef-derived adipocytes as described in other published studies. CONCLUSIONS: Pig-derived adipocytes expel large amounts of lipid directly into the medium environment prior to becoming capable of cell division, rather than retaining all lipids like the beef cells. This difference in lipid handling and trafficking may be a novel mechanism in adipocyte resumption of proliferation.
BACKGROUND AND OBJECTIVES: Numerous in vitro observations have been published to show that mature adipocytes may resume proliferation and begin to populate the adipofibroblast fraction or form other cell types. METHODS AND RESULTS: In the present study, we evaluated clonal cultures of mature pig-derived adipocytes as they began to reestablish their ability to divide. The lipid contained within the cytoplasm was either moved to the apical ends of the cell, or large droplets were physically extruded from the cell. In the latter case, we ascertained that the cell lipid droplet was handled in a different manner to that by beef-derived adipocytes as described in other published studies. CONCLUSIONS:Pig-derived adipocytes expel large amounts of lipid directly into the medium environment prior to becoming capable of cell division, rather than retaining all lipids like the beef cells. This difference in lipid handling and trafficking may be a novel mechanism in adipocyte resumption of proliferation.
Authors: G J Hausman; M V Dodson; K Ajuwon; M Azain; K M Barnes; L L Guan; Z Jiang; S P Poulos; R D Sainz; S Smith; M Spurlock; J Novakofski; M E Fernyhough; W G Bergen Journal: J Anim Sci Date: 2008-10-10 Impact factor: 3.159
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Authors: Shengjuan Wei; Marcio S Duarte; Linsen Zan; Min Du; Zhihua Jiang; LeLuo Guan; Jie Chen; Gary J Hausman; Michael V Dodson Journal: J Genomics Date: 2013-10-15