Youngsook Son1, Dong Sun Lee2, Ji Yeon Lee2, Guangfan Chi1. 1. College of Life Science, Kyung Hee University, Yongin and. 2. Laboratory of Tissue Engineering, Korea Institute of Radiological & Medical Sciences, Seoul, Korea.
Abstract
BACKGROUND AND OBJECTIVES: Neural stem/precursor cells are found in relatively inaccessible neurogenic regions of the adult brain, making them difficult to harvest for therapeutic purposes. METHODS AND RESULTS: In this study, nestin-expressing spheroids were induced from primary cultures of newborn and adult foreskin-derived dermal fibroblasts using a novel, two-step induction method. Approximately 80% of dermal fibroblasts became nestin-expressing cells within 12 days. Nestin expression and spheroid-forming capacity were both blocked by removal of bFGF from the induction medium. The bFGF-induced, nestin-expressing spheroids possessed most of the features distinctive of skin-derived precursor cells. CONCLUSIONS: Our findings support the possibility of inducing nestin-expressing spheroids from purified human dermal fibroblasts in a bFGF dependent manner. This is important because it may be impossible to induce spheroids from the small pool of residual neural crest-related precursor cells found in adults.
BACKGROUND AND OBJECTIVES: Neural stem/precursor cells are found in relatively inaccessible neurogenic regions of the adult brain, making them difficult to harvest for therapeutic purposes. METHODS AND RESULTS: In this study, nestin-expressing spheroids were induced from primary cultures of newborn and adult foreskin-derived dermal fibroblasts using a novel, two-step induction method. Approximately 80% of dermal fibroblasts became nestin-expressing cells within 12 days. Nestin expression and spheroid-forming capacity were both blocked by removal of bFGF from the induction medium. The bFGF-induced, nestin-expressing spheroids possessed most of the features distinctive of skin-derived precursor cells. CONCLUSIONS: Our findings support the possibility of inducing nestin-expressing spheroids from purified human dermal fibroblasts in a bFGF dependent manner. This is important because it may be impossible to induce spheroids from the small pool of residual neural crest-related precursor cells found in adults.
Authors: Karl J L Fernandes; Ian A McKenzie; Pleasantine Mill; Kristen M Smith; Mahnaz Akhavan; Fanie Barnabé-Heider; Jeff Biernaskie; Adrienne Junek; Nao R Kobayashi; Jean G Toma; David R Kaplan; Patricia A Labosky; Victor Rafuse; Chi-Chung Hui; Freda D Miller Journal: Nat Cell Biol Date: 2004-11 Impact factor: 28.824
Authors: Karl J L Fernandes; Nao R Kobayashi; Conor J Gallagher; Fanie Barnabé-Heider; Anne Aumont; David R Kaplan; Freda D Miller Journal: Exp Neurol Date: 2006-05-05 Impact factor: 5.330
Authors: J G Toma; M Akhavan; K J Fernandes; F Barnabé-Heider; A Sadikot; D R Kaplan; F D Miller Journal: Nat Cell Biol Date: 2001-09 Impact factor: 28.824
Authors: Christine E Wong; Christian Paratore; María T Dours-Zimmermann; Ariane Rochat; Thomas Pietri; Ueli Suter; Dieter R Zimmermann; Sylvie Dufour; Jean Paul Thiery; Dies Meijer; Friedrich Beermann; Yann Barrandon; Lukas Sommer Journal: J Cell Biol Date: 2006-12-11 Impact factor: 10.539