Regulation of diacylglycerol kinase δ2 expression in C2C12 skeletal muscle cells by free fatty acids.

Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles is closely related to the pathogenesis of type 2 diabetes. However, the regulation of DGKδ expression is not well understood. In this study, we found that myristic acid (14:0) significantly increased DGKδ2 protein expression in a dose-dependent manner (EC(50) = 0.16 mM) in mouse C2C12 myotubes. In contrast, oleic [18:1(n-9)], eicosenoic [20:1(n-9)] and erucic [22:1(n-9)] acids markedly decreased DGKδ2 expression. Myristic acid slowly enhanced DGKδ2 expression at the transcription level. Therefore, DGKδ2 expression is positively regulated by the relatively short-chain saturated fatty acid myristic acid but attenuated by n-9 monounsaturated fatty acids.