| Literature DB >> 24852216 |
Ahmad Daryani1, Mehdi Sharif2, Yousef Dadimoghaddam1, Mohammad Bagher Hashemi Souteh3, Ehsan Ahmadpour1, Alireza Khalilian4, Shahabeddin Sarvi1, Touraj Farazmand5, Hamed Kalani6, Mehdi Rasouli3.
Abstract
Excretory-secretory antigens (ESAs) of Toxoplasma gondii are one of the candidates for immunization against toxoplasmosis. For evaluation of immunization, we determined the kinetics of the distribution of Toxoplasma and parasite load in different tissues of mice immunized by ESAs. In this experimental study, 36 mice in case (n=18) and control (n=18) groups were immunized with ESAs and PBS, respectively. After 2weeks, mice were challenged intraperitoneally with Toxoplasma virulent RH strain. Blood and different tissues (brain, spleen, liver, heart, kidney, and muscle) were collected daily after challenge (1, 2, 3 and last day before death). Parasite load was calculated using Real time QPCR targeted at the B1 gene. ESAs as vaccine in different tissues showed various effects. However, infected mice which received the vaccine in comparison with control group, displayed a drastically decreasing in parasite burden, in their blood and tissues (P=0.000). These results indicated that ESAs with reduction of parasite load in different tissues of host could be evaluable candidate for the development of immunization strategies against toxoplasmosis.Entities:
Keywords: Excretory–secretory antigens; Parasite load; QPCR; Toxoplasmosis; Vaccine
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Year: 2014 PMID： 24852216 DOI： 10.1016/j.exppara.2014.05.008
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011