Literature DB >> 24850380

Polymerase synthesis of photocaged DNA resistant against cleavage by restriction endonucleases.

Zuzana Vaníková1, Michal Hocek.   

Abstract

5-[(2-Nitrobenzyl)oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  DNA; DNA polymerase; nucleotides; photocaging; protective groups

Mesh：

Substances：

Year:  2014        PMID： 24850380     DOI： 10.1002/anie.201402370

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  6 in total

1.  N4-acyl-2'-deoxycytidine-5'-triphosphates for the enzymatic synthesis of modified DNA.

Authors:  Jevgenija Jakubovska; Daiva Tauraite; Lukas Birštonas; Rolandas Meškys
Journal:  Nucleic Acids Res       Date:  2018-07-06       Impact factor: 16.971

2.  A Benzophenone-Based Photocaging Strategy for the N7 Position of Guanosine.

Authors:  Lea Anhäuser; Nils Klöcker; Fabian Muttach; Florian Mäsing; Petr Špaček; Armido Studer; Andrea Rentmeister
Journal:  Angew Chem Int Ed Engl       Date:  2019-12-30       Impact factor: 15.336

3.  Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases.

Authors:  Veronika Raindlová; Martina Janoušková; Michaela Slavíčková; Pavla Perlíková; Soňa Boháčová; Nemanja Milisavljevič; Hana Šanderová; Martin Benda; Ivan Barvík; Libor Krásný; Michal Hocek
Journal:  Nucleic Acids Res       Date:  2016-03-21       Impact factor: 16.971

4.  Azidophenyl as a click-transformable redox label of DNA suitable for electrochemical detection of DNA-protein interactions.

Authors:  Jana Balintová; Jan Špaček; Radek Pohl; Marie Brázdová; Luděk Havran; Miroslav Fojta; Michal Hocek
Journal:  Chem Sci       Date:  2014-09-16       Impact factor: 9.825

5.  2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA.

Authors:  Ján Matyašovský; Radek Pohl; Michal Hocek
Journal:  Chemistry       Date:  2018-09-06       Impact factor: 5.236

6.  In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines.

Authors:  Kiersten Flodman; Ivan R Corrêa; Nan Dai; Peter Weigele; Shuang-Yong Xu
Journal:  Front Microbiol       Date:  2020-10-27       Impact factor: 5.640
  6 in total

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