Literature DB >> 2484754

Biochemical studies of human (A549) and simian (CV1) cells labeled with 64Cu, 67Cu, and 125IUdR.

J Grisvard1, E Guille, I Sasaki, I Sissoeff, A Valenza, S Apelgot, J Coppey, A Gaudemer.   

Abstract

CV1 and A549 cells were grown in the presence of 64Cu porphyric complex, 64CuCl2, or 67CuCl2. Radioactive copper determinations were performed on whole cells and on isolated cellular DNA. 125IUdR was used to calibrate the particular extraction and purification procedures we developed because of the half-lives of 64Cu and 67Cu. The results obtained have shown that some radioactive copper atoms remained firmly bound to the DNA molecule. Their amount was of the same order when using two different DNA isolation methods for the two cell lines studied. No significant differences were found when 64Cu was used as CuCl2 or as porphyric complex.

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Year:  1989        PMID: 2484754     DOI: 10.1007/bf02917436

Source DB:  PubMed          Journal:  Biol Trace Elem Res        ISSN: 0163-4984            Impact factor:   3.738


  13 in total

1.  PREPARATION AND PROPERTIES OF NATIVE CRAB DAT.

Authors:  N DAVIDSON; J WIDHOLM; U S NANDI; R JENSEN; B M OLIVERA; J C WANG
Journal:  Proc Natl Acad Sci U S A       Date:  1965-01       Impact factor: 11.205

2.  The radiotoxicity of iodine-125 in mammalian cells. I. Effects on the survival curve of radioiodine incorporated into DNA.

Authors:  E W Bradley; P C Chan; S J Adelstein
Journal:  Radiat Res       Date:  1975-12       Impact factor: 2.841

Review 3.  Studies on metal ions-DNA interactions: specific behaviour of reiterative DNA sequences.

Authors:  I Sissoëff; J Grisvard; E Guillé
Journal:  Prog Biophys Mol Biol       Date:  1976       Impact factor: 3.667

4.  Content of some trace elements in sarcoma M-1 DNA in dynamics of malignant growth.

Authors:  E L Andronikashvili; L M Mosulishvili; A I Belokobilski; N E Kharabadze; T K Tevzieva; E Y Efremova
Journal:  Cancer Res       Date:  1974-02       Impact factor: 12.701

5.  Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and nucleotides.

Authors:  R M Izatt; J J Christensen; J H Rytting
Journal:  Chem Rev       Date:  1971-10       Impact factor: 60.622

6.  The interaction of metal ions with polynucleotides and related compounds. 13. The effect of metal ions on the enzymatic degradation of ribonucleic acid by bovine pancreatic ribonuclease and of deoxyribonucleic acid by bovine pancreatic deoxyribonuclease I.

Authors:  G L Eichhorn; P Clark; E Tarien
Journal:  J Biol Chem       Date:  1969-02-10       Impact factor: 5.157

7.  Radiotoxicity of intranuclear tritium, 125 iodine and 131 iodine.

Authors:  K G Hofer; W L Hughes
Journal:  Radiat Res       Date:  1971-07       Impact factor: 2.841

8.  [Lethal effect of the decay of 64Cu and 67Cu incorporated in mammalian cells].

Authors:  S Apelgot; J Coppey; J Grisvard; E Guillé; I Sissoëff
Journal:  C R Acad Sci III       Date:  1984

9.  Dose-dependent preferential binding of polycyclic aromatic hydrocarbons to reiterated DNA of murine skin cells in culture.

Authors:  M Shoyab
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

10.  Distribution of copper-64 in control mice and in mice bearing ascitic Krebs tumor cells.

Authors:  S Apelgot; J Coppey; J Grisvard; E Guillé; I Sissoëff
Journal:  Cancer Res       Date:  1981-04       Impact factor: 12.701

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