| Literature DB >> 24847036 |
Suzanne M Johnson1, Erin L Carlson2, Frederick S Fisher3, Demosthenes Pappagianis2.
Abstract
Soil samples were collected in 2006 from Dinosaur National Monument (DNM), Utah, the site of an outbreak of coccidioidomycosis in 2001. DNA was isolated from two soil samples, and polymerase chain reaction (PCR) amplified Coccidioides DNA present in both samples. Ribosomal RNA genes and internal transcribed spacer (ITS) region PCR products were sequenced. Single-nucleotide polymorphisms indicated that the DNA from sample SS06RH was that of Coccidioides immitis, while the DNA from sample SS06UM was C. posadasii. This is the first report to directly demonstrate Coccidioides in soils from DNM and the first to report the presence of both C. immitis and C. posadasii in the same geographic location.Entities:
Keywords: Coccidioides immitis; Coccidioides posadasii; DNA; ITS; PCR; polymorphism
Mesh:
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Year: 2014 PMID: 24847036 DOI: 10.1093/mmy/myu004
Source DB: PubMed Journal: Med Mycol ISSN: 1369-3786 Impact factor: 4.076