Jingpeng Jin1, Shanshan Zhou2, Changfeng Li1, Ruisi Xu1, Lingling Zu3, Jiacong You4, Bin Zhang5. 1. Endoscopy Center China-Japan Union Hospital of Jilin University, 126 Xiantai Street, Changchun 130033, China. 2. The First Hospital of Jilin University, 71 Xinmin Street, Changchun 130021, China. 3. Tianjin Key Labotatory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China. 4. Tianjin Key Labotatory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address: yyjjcc_nk@163.com. 5. Endoscopy Center China-Japan Union Hospital of Jilin University, 126 Xiantai Street, Changchun 130033, China. Electronic address: zhangbin_j@163.com.
Abstract
AIMS: Aberrant expression of microRNAs (miRNAs) results in alterations of various biological processes (e.g., cell cycle, cell differentiation, and apoptosis) and cell transformation. Altered miRNAs expression was associated with lung carcinogenesis and tumor progression. This study aimed to investigate the function and underlying molecular events of miR-517a-3p on regulation of lung cancer cell proliferation and invasion. MAIN METHODS: Transfected miR-517a-3p mimics or inhibitors into 95D and 95C cells respectively, the effects of miR-517a-3p on lung cancer cell proliferation, migration, and invasion were detected. Bioinformatics software forecasted potential target genes of miR-517a-3p and dual luciferase reporter gene system and western blot verified whether miR-517a-3p regulates FOXJ3 expression directly. KEY FINDINGS: MiR-517a-3p was differentially expressed in lung cancer 95D and 95C cell lines that have different metastatic potential. Manipulation of miR-517a-3p expression changed lung cancer cell proliferation, migration and invasion capacity. MiR-517a-3p directly regulated FOXJ3 expression by binding to FOXJ3 promoter. SIGNIFICANCE: This study demonstrated that miR-517a-3p promoted lung cancer cell proliferation and invasion by targeting of FOXJ3 expression.
AIMS: Aberrant expression of microRNAs (miRNAs) results in alterations of various biological processes (e.g., cell cycle, cell differentiation, and apoptosis) and cell transformation. Altered miRNAs expression was associated with lung carcinogenesis and tumor progression. This study aimed to investigate the function and underlying molecular events of miR-517a-3p on regulation of lung cancer cell proliferation and invasion. MAIN METHODS: Transfected miR-517a-3p mimics or inhibitors into 95D and 95C cells respectively, the effects of miR-517a-3p on lung cancer cell proliferation, migration, and invasion were detected. Bioinformatics software forecasted potential target genes of miR-517a-3p and dual luciferase reporter gene system and western blot verified whether miR-517a-3p regulates FOXJ3 expression directly. KEY FINDINGS:MiR-517a-3p was differentially expressed in lung cancer 95D and 95C cell lines that have different metastatic potential. Manipulation of miR-517a-3p expression changed lung cancer cell proliferation, migration and invasion capacity. MiR-517a-3p directly regulated FOXJ3 expression by binding to FOXJ3 promoter. SIGNIFICANCE: This study demonstrated that miR-517a-3p promoted lung cancer cell proliferation and invasion by targeting of FOXJ3 expression.
Authors: Gati K Panigrahi; Anand Ramteke; Diane Birks; Hamdy E Abouzeid Ali; Sujatha Venkataraman; Chapla Agarwal; Rajeev Vibhakar; Lance D Miller; Rajesh Agarwal; Zakaria Y Abd Elmageed; Gagan Deep Journal: Oncotarget Date: 2018-02-17
Authors: Arlet María Acanda de la Rocha; Marisol González-Huarriz; Elizabeth Guruceaga; Nicole Mihelson; Sonia Tejada-Solís; Ricardo Díez-Valle; Naiara Martínez-Vélez; Juan Fueyo; Candelaria Gomez-Manzano; Marta M Alonso; John Laterra; Hernando López-Bertoni Journal: Arch Clin Biomed Res Date: 2020-06-18