| Literature DB >> 24846137 |
Yuichiro Enoki1, Tsuyoshi Sato2, Shinya Tanaka3, Takanori Iwata4, Michihiko Usui5, Shu Takeda6, Shoichiro Kokabu1, Masahito Matsumoto7, Masahiko Okubo8, Keisuke Nakashima5, Masayuki Yamato4, Teruo Okano4, Toru Fukuda6, Dai Chida1, Yuuki Imai9, Hisataka Yasuda10, Tatsuji Nishihara11, Masumi Akita12, Hiromi Oda3, Yasushi Okazaki7, Tatsuo Suda7, Tetsuya Yoda1.
Abstract
Bone is a highly vascularized organ, thus angiogenesis is a vital process during bone remodeling. However, the role of vascular systems in bone remodeling is not well recognized. Here we show that netrin-4 inhibits osteoclast differentiation in vitro and in vivo. Co-cultures of bone marrow macrophages with vascular endothelial cells markedly inhibited osteoclast differentiation. Adding a neutralizing antibody, or RNA interference against netrin-4, restored in vitro osteoclast differentiation. Administration of netrin-4 prevented bone loss in an osteoporosis mouse model by decreasing the osteoclast number. We propose that vascular endothelial cells interact with bone in suppressing bone through netrin-4.Entities:
Keywords: Inhibition; Netrin-4; Osteoclast; Vascular endothelial cell
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Year: 2014 PMID： 24846137 DOI： 10.1016/j.febslet.2014.05.009
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124