Literature DB >> 24845469

Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes.

Y Seesao1, C Audebert2, V Verrez-Bagnis3, S Merlin2, M Jérôme3, E Viscogliosi4, E Dei-Cas4, C M Aliouat-Denis5, M Gay6.   

Abstract

Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
Copyright © 2014. Published by Elsevier B.V.

Keywords:  Anisakidae; COX2; High-throughput sequencing; Marine fish; Nematode; Pooled DNA extraction

Mesh：

Substances：

Year:  2014        PMID： 24845469     DOI： 10.1016/j.mimet.2014.05.004

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

1.  Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes.

Authors:  S Sloan; C J Jenvey; D Piedrafita; S Preston; M J Stear
Journal:  BMC Biotechnol       Date:  2021-05-17       Impact factor: 2.563

2.  Real-time detection and identification of nematode eggs genus and species through optical imaging.

Authors:  Farah Qazi; Asma Khalid; Arpita Poddar; Jean-Philippe Tetienne; Athavan Nadarajah; Arturo Aburto-Medina; Esmaeil Shahsavari; Ravi Shukla; Steven Prawer; Andrew S Ball; Snjezana Tomljenovic-Hanic
Journal:  Sci Rep       Date:  2020-04-29       Impact factor: 4.379

3.  [New eating habits, new parasitic risks: The example of fish].

Authors:  J Dupouy-Camet; M Gay; R Houin
Journal:  Bull Acad Natl Med       Date:  2020-10-13       Impact factor: 0.144

4.  The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal.

Authors:  Matthew R Lee; Cristian B Canales-Aguirre; Daniela Nuñez; Karla Pérez; Crisitan E Hernández; Antonio Brante
Journal:  PLoS One       Date:  2017-10-05       Impact factor: 3.240
  4 in total

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