Jason Chia-Hsun Hsieh1,2, Hung-Chih Lin3, Chi-Ya Huang4, Hsung-Ling Hsu4, Tyler Min-Hsien Wu5, Chia-Lin Lee6,7, Min-Chi Chen8, Hung-Ming Wang1,9, Ching-Ping Tseng3,4,10. 1. Division of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taiwan, Republic of China. 2. Department of Chemical and Materials Engineering, Chang Gung University, Taiwan, Republic of China. 3. Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Taiwan, Republic of China. 4. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taiwan, Republic of China. 5. Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taiwan, Republic of China. 6. Division of Endocrinology and Metabolism, Department of Internal Medicine, Taichung Veterans General Hospital, Taiwan, Republic of China. 7. Department of Public Health, College of Public Health, China Medical University, Taiwan, Republic of China. 8. Department of Public Health and Biostatistics Consulting Center, College of Medicine, Chang Gung University, Taiwan, Republic of China. 9. Department of Medicine, College of Medicine, Chang Gung University, Taiwan, Republic of China. 10. Molecular Medicine Research Center, Chang Gung University, Taiwan, Republic of China.
Abstract
BACKGROUND: Podoplanin (PDPN) is a prognostic factor for head and neck squamous cell carcinoma (HNSCC). However, PDPN expression in circulating tumor cells (CTCs) and its prognostic value are not clear. METHODS: The PowerMag system was used to enumerate CTCs from 53 patients with HNSCC prechemotherapy and 61 healthy donors. PDPN expression was determined by immunofluorescence staining. Results were correlated with clinicopathological parameters and clinical outcome, such as patient survival by receiver operating characteristic (ROC) and univariate and multivariate analyses. RESULTS: PDPN was expressed in a subset of CTCs. Both EpCAM-positive CTC and PDPN-positive CTC counts were statistically different between the disease and nondisease groups (p < .0001) with no prognostic value. After a median follow-up of 10.5 months (range, 6.6-18.5 months), the PDPN-positive/EpCAM-positive CTC ratio >20% was a significant prognostic factor for death within 6 months (p = .011) and was correlated with poor progression-free survival (p = .016) and overall survival (p = .015). CONCLUSION: PDPN-positive/EpCAM-positive CTC ratio is a prognostic factor and defining the ratio in patients with HNSCC might be valuable to clinical management.
BACKGROUND:Podoplanin (PDPN) is a prognostic factor for head and neck squamous cell carcinoma (HNSCC). However, PDPN expression in circulating tumor cells (CTCs) and its prognostic value are not clear. METHODS: The PowerMag system was used to enumerate CTCs from 53 patients with HNSCC prechemotherapy and 61 healthy donors. PDPN expression was determined by immunofluorescence staining. Results were correlated with clinicopathological parameters and clinical outcome, such as patient survival by receiver operating characteristic (ROC) and univariate and multivariate analyses. RESULTS:PDPN was expressed in a subset of CTCs. Both EpCAM-positive CTC and PDPN-positive CTC counts were statistically different between the disease and nondisease groups (p < .0001) with no prognostic value. After a median follow-up of 10.5 months (range, 6.6-18.5 months), the PDPN-positive/EpCAM-positive CTC ratio >20% was a significant prognostic factor for death within 6 months (p = .011) and was correlated with poor progression-free survival (p = .016) and overall survival (p = .015). CONCLUSION:PDPN-positive/EpCAM-positive CTC ratio is a prognostic factor and defining the ratio in patients with HNSCC might be valuable to clinical management.
Authors: Vasudha Mishra; Alka Singh; Xiangying Chen; Ari J Rosenberg; Alexander T Pearson; Alex Zhavoronkov; Peter A Savage; Mark W Lingen; Nishant Agrawal; Evgeny Izumchenko Journal: Br J Cancer Date: 2021-12-07 Impact factor: 7.640
Authors: Johanna Inhestern; Katrin Oertel; Viola Stemmann; Harald Schmalenberg; Andreas Dietz; Nicole Rotter; Johannes Veit; Martin Görner; Holger Sudhoff; Christian Junghanß; Claus Wittekindt; Katharina Pachmann; Orlando Guntinas-Lichius Journal: PLoS One Date: 2015-07-17 Impact factor: 3.240