Literature DB >> 24841298

Yeast transformation by the LiAc/SS carrier DNA/PEG method.

R Daniel Gietz1.   

Abstract

Transformation is essential to many molecular and genetic investigations in the yeast Saccharomyces cerevisiae. Yeast transformation protocols utilizing the LiAc/ssDNA/PEG method are presented. Protocols for various applications are listed including a method for transformation in 96-well microtiter plate format and another for the production of frozen competent yeast cells that can be used at a moment's notice.

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Year:  2014        PMID： 24841298     DOI： 10.1007/978-1-4939-0799-1_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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