Yu Chen1, Xuefen Li2, Li Tian2, Shufa Zheng2, Shigui Yang1, Yuejiao Dong2, Yiyin Wang2, Dawei Cui2, Xiaoli Liu1, Weifeng Liang1, Honglin Chen3, Lanjuan Li4. 1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, PR China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, PR China. 2. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, PR China. 3. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, PR China; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Hong Kong SAR, PR China. 4. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, PR China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, PR China. Electronic address: ljli@zju.edu.cn.
Abstract
OBJECTIVES: To investigate peripheral blood lymphocyte subgroups response to the H7N9 virus and identify potential correlations between the anti-viral response and clinical outcomes in infected patients. METHODS: T lymphocyte subgroups, cytokine/chemokine levels in peripheral blood and H7N9 viral loads in the sputum were measured for 53 H7N9 patients (14 lethal and 39 non-lethal cases). 22 H1N1 patients and 15 healthy volunteers were selected as controls. RESULTS: Low proportions of T cells were observed in H7N9-infected individuals, particularly those who later died, and these correlated with clinical APACHE II scores and H7N9 virus loads in sputum. T-cell levels fluctuated during hospitalization and decreased suddenly on the day of death in those who succumbed to infection, whereas a dramatic increase in lymphocyte subgroups was observed in those who survived beyond the early stage of infection, with the levels of most lymphocyte subgroups significantly higher during the recovery phase compared to the early phase of infection. A cytokine/chemokine storm was also confirmed in this study. CONCLUSIONS: H7N9-infected individuals have low proportions of peripheral blood T lymphocyte subgroups, particularly those suffering fatal infections. The numbers of CD3(+) T cells (including CD4(+) and CD8(+) T cells) may predict the clinical outcome of human H7N9 infection.
OBJECTIVES: To investigate peripheral blood lymphocyte subgroups response to the H7N9 virus and identify potential correlations between the anti-viral response and clinical outcomes in infectedpatients. METHODS: T lymphocyte subgroups, cytokine/chemokine levels in peripheral blood and H7N9 viral loads in the sputum were measured for 53 H7N9patients (14 lethal and 39 non-lethal cases). 22 H1N1patients and 15 healthy volunteers were selected as controls. RESULTS: Low proportions of T cells were observed in H7N9-infected individuals, particularly those who later died, and these correlated with clinical APACHE II scores and H7N9 virus loads in sputum. T-cell levels fluctuated during hospitalization and decreased suddenly on the day of death in those who succumbed to infection, whereas a dramatic increase in lymphocyte subgroups was observed in those who survived beyond the early stage of infection, with the levels of most lymphocyte subgroups significantly higher during the recovery phase compared to the early phase of infection. A cytokine/chemokine storm was also confirmed in this study. CONCLUSIONS:H7N9-infected individuals have low proportions of peripheral blood T lymphocyte subgroups, particularly those suffering fatal infections. The numbers of CD3(+) T cells (including CD4(+) and CD8(+) T cells) may predict the clinical outcome of humanH7N9infection.
Authors: William J Liu; Haixia Xiao; Lianpan Dai; Di Liu; Jianjun Chen; Xiaopeng Qi; Yuhai Bi; Yi Shi; George F Gao; Yingxia Liu Journal: Front Med Date: 2021-04-16 Impact factor: 4.592